Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray
(2003) In British Journal of Cancer 88(9). p.1417-1423- Abstract
- Aberrations in the GI/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of GI/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D 1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D I and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed... (More)
- Aberrations in the GI/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of GI/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D 1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D I and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of GI/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin DI protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that GI/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes. (C) 2003 Cancer Research UK. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/310719
- author
- Hedberg, Y ; Ljungberg, B ; Roos, G and Landberg, Göran LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- renal, protein, tissue microarray, cyclin, cell cycle, G1/S transition, cell carcinoma
- in
- British Journal of Cancer
- volume
- 88
- issue
- 9
- pages
- 1417 - 1423
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000183025200016
- pmid:12778072
- scopus:0038823526
- pmid:12778072
- ISSN
- 1532-1827
- DOI
- 10.1038/sj.bjc.6600922
- language
- English
- LU publication?
- yes
- id
- ccc43741-e4ca-40e9-9c4d-5383263ba864 (old id 310719)
- date added to LUP
- 2016-04-01 11:53:38
- date last changed
- 2022-04-28 21:34:03
@article{ccc43741-e4ca-40e9-9c4d-5383263ba864,
abstract = {{Aberrations in the GI/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of GI/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D 1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D I and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of GI/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin DI protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that GI/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes. (C) 2003 Cancer Research UK.}},
author = {{Hedberg, Y and Ljungberg, B and Roos, G and Landberg, Göran}},
issn = {{1532-1827}},
keywords = {{renal; protein; tissue microarray; cyclin; cell cycle; G1/S transition; cell carcinoma}},
language = {{eng}},
number = {{9}},
pages = {{1417--1423}},
publisher = {{Nature Publishing Group}},
series = {{British Journal of Cancer}},
title = {{Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray}},
url = {{http://dx.doi.org/10.1038/sj.bjc.6600922}},
doi = {{10.1038/sj.bjc.6600922}},
volume = {{88}},
year = {{2003}},
}