Kinetics of somatic mutation in lymph node germinal centres
(1994) In Scandinavian Journal of Immunology 40(5). p.80-469- Abstract
Somatic mutation activity in immunoglobulin V kappa genes during the response to the hapten 2-phenyl-5-oxazolone was measured in lymph node B-cell populations at various timepoints after footpad immunization. When the V kappa Ox1 genes rearranged to the J kappa 5 segment were amplified from genomic DNA using the polymerase chain reaction and sequenced, somatic mutations could be detected as early as day 4 after immunization. Somatic mutations were also detected after sequencing RNA from oxazolone-specific hybridomas derived from lymph node cells at day 4 after immunization. These early mutations were found mostly in cells with a germinal centre phenotype. No indication of selection at the population level by apoptosis was detected until... (More)
Somatic mutation activity in immunoglobulin V kappa genes during the response to the hapten 2-phenyl-5-oxazolone was measured in lymph node B-cell populations at various timepoints after footpad immunization. When the V kappa Ox1 genes rearranged to the J kappa 5 segment were amplified from genomic DNA using the polymerase chain reaction and sequenced, somatic mutations could be detected as early as day 4 after immunization. Somatic mutations were also detected after sequencing RNA from oxazolone-specific hybridomas derived from lymph node cells at day 4 after immunization. These early mutations were found mostly in cells with a germinal centre phenotype. No indication of selection at the population level by apoptosis was detected until day 7 after immunization. These results suggest somatic mutations can be induced very early during the immune response in lymph node cells, prior to the peak of clonal expansion and selection with regard to antigen binding.
(Less)
- author
- Källberg, E LU ; Gray, D and Leanderson, T LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Apoptosis/genetics, B-Lymphocytes/immunology, Base Sequence, Cells, Cultured, DNA/genetics, Gene Rearrangement, B-Lymphocyte/genetics, Haptens/immunology, Histocompatibility Antigens Class II, Immunoenzyme Techniques, Immunoglobulin kappa-Chains/genetics, Lymph Nodes/cytology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Molecular Sequence Data, Mutation/immunology, Oxazolone/analogs & derivatives, Spleen/cytology
- in
- Scandinavian Journal of Immunology
- volume
- 40
- issue
- 5
- pages
- 80 - 469
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028077591
- pmid:7973453
- ISSN
- 0300-9475
- DOI
- 10.1111/j.1365-3083.1994.tb03492.x
- language
- English
- LU publication?
- yes
- id
- cd06ef7a-dd61-4487-8416-390a51cc7786
- date added to LUP
- 2021-10-07 10:51:14
- date last changed
- 2024-01-05 17:57:44
@article{cd06ef7a-dd61-4487-8416-390a51cc7786,
abstract = {{<p>Somatic mutation activity in immunoglobulin V kappa genes during the response to the hapten 2-phenyl-5-oxazolone was measured in lymph node B-cell populations at various timepoints after footpad immunization. When the V kappa Ox1 genes rearranged to the J kappa 5 segment were amplified from genomic DNA using the polymerase chain reaction and sequenced, somatic mutations could be detected as early as day 4 after immunization. Somatic mutations were also detected after sequencing RNA from oxazolone-specific hybridomas derived from lymph node cells at day 4 after immunization. These early mutations were found mostly in cells with a germinal centre phenotype. No indication of selection at the population level by apoptosis was detected until day 7 after immunization. These results suggest somatic mutations can be induced very early during the immune response in lymph node cells, prior to the peak of clonal expansion and selection with regard to antigen binding.</p>}},
author = {{Källberg, E and Gray, D and Leanderson, T}},
issn = {{0300-9475}},
keywords = {{Animals; Apoptosis/genetics; B-Lymphocytes/immunology; Base Sequence; Cells, Cultured; DNA/genetics; Gene Rearrangement, B-Lymphocyte/genetics; Haptens/immunology; Histocompatibility Antigens Class II; Immunoenzyme Techniques; Immunoglobulin kappa-Chains/genetics; Lymph Nodes/cytology; Mice; Mice, Inbred BALB C; Mice, Transgenic; Molecular Sequence Data; Mutation/immunology; Oxazolone/analogs & derivatives; Spleen/cytology}},
language = {{eng}},
number = {{5}},
pages = {{80--469}},
publisher = {{Wiley-Blackwell}},
series = {{Scandinavian Journal of Immunology}},
title = {{Kinetics of somatic mutation in lymph node germinal centres}},
url = {{http://dx.doi.org/10.1111/j.1365-3083.1994.tb03492.x}},
doi = {{10.1111/j.1365-3083.1994.tb03492.x}},
volume = {{40}},
year = {{1994}},
}