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Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast

Ding, Bao Jian LU ; Wang, Hong Lei LU ; Al-Saleh, Mohammed Ali ; Löfstedt, Christer LU and Antony, Binu LU (2022) In Pest Management Science 78(3). p.1048-1059
Abstract

BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using... (More)

BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(Z,E)-9,12-tetradecadienyl acetate, bioproduction of pheromone, mating disruption, pest management, pheromone, Saccharomyces cerevisiae
in
Pest Management Science
volume
78
issue
3
pages
1048 - 1059
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85120555207
  • pmid:34773383
ISSN
1526-498X
DOI
10.1002/ps.6716
project
OLEFINE: OLEaginous yeast platforms for FINE chemicals
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021 Society of Chemical Industry.
id
cd15ee9f-09d2-47ef-b3a7-07f2d8ff3ace
date added to LUP
2022-01-26 10:54:05
date last changed
2024-06-16 00:29:55
@article{cd15ee9f-09d2-47ef-b3a7-07f2d8ff3ace,
  abstract     = {{<p>BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.</p>}},
  author       = {{Ding, Bao Jian and Wang, Hong Lei and Al-Saleh, Mohammed Ali and Löfstedt, Christer and Antony, Binu}},
  issn         = {{1526-498X}},
  keywords     = {{(Z,E)-9,12-tetradecadienyl acetate; bioproduction of pheromone; mating disruption; pest management; pheromone; Saccharomyces cerevisiae}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1048--1059}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Pest Management Science}},
  title        = {{Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast}},
  url          = {{http://dx.doi.org/10.1002/ps.6716}},
  doi          = {{10.1002/ps.6716}},
  volume       = {{78}},
  year         = {{2022}},
}