Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast
(2022) In Pest Management Science 78(3). p.1048-1059- Abstract
BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using... (More)
BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.
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- author
- Ding, Bao Jian LU ; Wang, Hong Lei LU ; Al-Saleh, Mohammed Ali ; Löfstedt, Christer LU and Antony, Binu LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (Z,E)-9,12-tetradecadienyl acetate, bioproduction of pheromone, mating disruption, pest management, pheromone, Saccharomyces cerevisiae
- in
- Pest Management Science
- volume
- 78
- issue
- 3
- pages
- 1048 - 1059
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:34773383
- scopus:85120555207
- ISSN
- 1526-498X
- DOI
- 10.1002/ps.6716
- project
- OLEFINE: OLEaginous yeast platforms for FINE chemicals
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021 Society of Chemical Industry.
- id
- cd15ee9f-09d2-47ef-b3a7-07f2d8ff3ace
- date added to LUP
- 2022-01-26 10:54:05
- date last changed
- 2024-09-08 08:29:57
@article{cd15ee9f-09d2-47ef-b3a7-07f2d8ff3ace, abstract = {{<p>BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.</p>}}, author = {{Ding, Bao Jian and Wang, Hong Lei and Al-Saleh, Mohammed Ali and Löfstedt, Christer and Antony, Binu}}, issn = {{1526-498X}}, keywords = {{(Z,E)-9,12-tetradecadienyl acetate; bioproduction of pheromone; mating disruption; pest management; pheromone; Saccharomyces cerevisiae}}, language = {{eng}}, number = {{3}}, pages = {{1048--1059}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Pest Management Science}}, title = {{Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast}}, url = {{http://dx.doi.org/10.1002/ps.6716}}, doi = {{10.1002/ps.6716}}, volume = {{78}}, year = {{2022}}, }