Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
(2019) In European Journal of Immunology 49(10). p.1457-1973- Abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this... (More)
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/cd1aed89-869c-4826-a2f5-e516a06a284a
- author
- Cossarizza, Andrea ; Agace, William LU and Zychlinsky, A.
- author collaboration
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- animal cell, animal experiment, article, cell selection, consensus, flow cytometry, immunocompetent cell, immunologist, immunology, nonhuman, phenotype, practice guideline
- in
- European Journal of Immunology
- volume
- 49
- issue
- 10
- pages
- 517 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:31633216
- scopus:85073598520
- ISSN
- 1521-4141
- DOI
- 10.1002/eji.201970107
- language
- English
- LU publication?
- yes
- id
- cd1aed89-869c-4826-a2f5-e516a06a284a
- date added to LUP
- 2019-12-17 08:52:28
- date last changed
- 2022-04-18 19:28:16
@article{cd1aed89-869c-4826-a2f5-e516a06a284a, abstract = {{These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim}}, author = {{Cossarizza, Andrea and Agace, William and Zychlinsky, A.}}, issn = {{1521-4141}}, keywords = {{animal cell; animal experiment; article; cell selection; consensus; flow cytometry; immunocompetent cell; immunologist; immunology; nonhuman; phenotype; practice guideline}}, language = {{eng}}, number = {{10}}, pages = {{1457--1973}}, publisher = {{John Wiley & Sons Inc.}}, series = {{European Journal of Immunology}}, title = {{Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)}}, url = {{http://dx.doi.org/10.1002/eji.201970107}}, doi = {{10.1002/eji.201970107}}, volume = {{49}}, year = {{2019}}, }