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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza, Andrea ; Agace, William LU and Zychlinsky, A. (2019) In European Journal of Immunology 49(10). p.1457-1973
Abstract
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this... (More)
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim (Less)
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author
author collaboration
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
animal cell, animal experiment, article, cell selection, consensus, flow cytometry, immunocompetent cell, immunologist, immunology, nonhuman, phenotype, practice guideline
in
European Journal of Immunology
volume
49
issue
10
pages
517 pages
publisher
John Wiley and Sons Inc.
external identifiers
  • pmid:31633216
  • scopus:85073598520
ISSN
1521-4141
DOI
10.1002/eji.201970107
language
English
LU publication?
yes
id
cd1aed89-869c-4826-a2f5-e516a06a284a
date added to LUP
2019-12-17 08:52:28
date last changed
2020-06-03 05:14:58
@article{cd1aed89-869c-4826-a2f5-e516a06a284a,
  abstract     = {These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},
  author       = {Cossarizza, Andrea and Agace, William and Zychlinsky, A.},
  issn         = {1521-4141},
  language     = {eng},
  number       = {10},
  pages        = {1457--1973},
  publisher    = {John Wiley and Sons Inc.},
  series       = {European Journal of Immunology},
  title        = {Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)},
  url          = {http://dx.doi.org/10.1002/eji.201970107},
  doi          = {10.1002/eji.201970107},
  volume       = {49},
  year         = {2019},
}