Investigation of graphite electrodes modified with cellobiose dehydrogenase from the ascomycete Myriococcum thermophilum
(2007) In Electroanalysis 19(2-3). p.172-180- Abstract
- The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence... (More)
- The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence and presence of the mediator 1,4-benzoquinone in the presence of cellobiose or lactose. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/674666
- author
- Harreither, Wolfgang ; Coman, Vasile LU ; Ludwig, Roland ; Haltrich, Dietmar and Gorton, Lo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cellobiose dehydrogenase, flow injection analysis, biosensor, carbohydrate, direct electron transfer, mediated electron transfer
- in
- Electroanalysis
- volume
- 19
- issue
- 2-3
- pages
- 172 - 180
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000244220700008
- scopus:34547587876
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.200603688
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- cd343a1c-e845-4498-b6a6-d5bb48cdfb7d (old id 674666)
- date added to LUP
- 2016-04-01 15:20:47
- date last changed
- 2022-02-12 07:33:25
@article{cd343a1c-e845-4498-b6a6-d5bb48cdfb7d,
abstract = {{The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence and presence of the mediator 1,4-benzoquinone in the presence of cellobiose or lactose.}},
author = {{Harreither, Wolfgang and Coman, Vasile and Ludwig, Roland and Haltrich, Dietmar and Gorton, Lo}},
issn = {{1040-0397}},
keywords = {{cellobiose dehydrogenase; flow injection analysis; biosensor; carbohydrate; direct electron transfer; mediated electron transfer}},
language = {{eng}},
number = {{2-3}},
pages = {{172--180}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electroanalysis}},
title = {{Investigation of graphite electrodes modified with cellobiose dehydrogenase from the ascomycete Myriococcum thermophilum}},
url = {{http://dx.doi.org/10.1002/elan.200603688}},
doi = {{10.1002/elan.200603688}},
volume = {{19}},
year = {{2007}},
}