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Cytotoxicity induced by mixtures of adenovirus and specific antibodies - Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera

Ankerst, J. LU orcid and Kjellén, L. (1973) In Archiv für die gesamte Virusforschung 41(1-2). p.99-105
Abstract

Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a... (More)

Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower51Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archiv für die gesamte Virusforschung
volume
41
issue
1-2
pages
99 - 105
publisher
Springer
external identifiers
  • pmid:4736810
  • scopus:0015537941
ISSN
0003-9012
DOI
10.1007/BF01249934
language
English
LU publication?
yes
id
cd40796a-f84c-4660-893b-396d47a63cc8
date added to LUP
2021-01-28 17:18:56
date last changed
2024-01-03 05:50:36
@article{cd40796a-f84c-4660-893b-396d47a63cc8,
  abstract     = {{<p>Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower<sup>51</sup>Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.</p>}},
  author       = {{Ankerst, J. and Kjellén, L.}},
  issn         = {{0003-9012}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{99--105}},
  publisher    = {{Springer}},
  series       = {{Archiv für die gesamte Virusforschung}},
  title        = {{Cytotoxicity induced by mixtures of adenovirus and specific antibodies - Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera}},
  url          = {{http://dx.doi.org/10.1007/BF01249934}},
  doi          = {{10.1007/BF01249934}},
  volume       = {{41}},
  year         = {{1973}},
}