Cytotoxicity induced by mixtures of adenovirus and specific antibodies - Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera
(1973) In Archiv für die gesamte Virusforschung 41(1-2). p.99-105- Abstract
Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a... (More)
Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower51Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.
(Less)
- author
- Ankerst, J. LU and Kjellén, L.
- organization
- publishing date
- 1973
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Archiv für die gesamte Virusforschung
- volume
- 41
- issue
- 1-2
- pages
- 99 - 105
- publisher
- Springer
- external identifiers
-
- pmid:4736810
- scopus:0015537941
- ISSN
- 0003-9012
- DOI
- 10.1007/BF01249934
- language
- English
- LU publication?
- yes
- id
- cd40796a-f84c-4660-893b-396d47a63cc8
- date added to LUP
- 2021-01-28 17:18:56
- date last changed
- 2024-01-03 05:50:36
@article{cd40796a-f84c-4660-893b-396d47a63cc8, abstract = {{<p>Aggregates cytotoxie for human cells in vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower<sup>51</sup>Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.</p>}}, author = {{Ankerst, J. and Kjellén, L.}}, issn = {{0003-9012}}, language = {{eng}}, number = {{1-2}}, pages = {{99--105}}, publisher = {{Springer}}, series = {{Archiv für die gesamte Virusforschung}}, title = {{Cytotoxicity induced by mixtures of adenovirus and specific antibodies - Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera}}, url = {{http://dx.doi.org/10.1007/BF01249934}}, doi = {{10.1007/BF01249934}}, volume = {{41}}, year = {{1973}}, }