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Chlorophenol stress affects aromatic amino acid biosynthesis-a genome-wide study

Yadav, Vikas LU ; Shitiz, Kirti ; Pandey, Rishi and Yadav, Jyoti (2010) In Yeast 28(1). p.81-91
Abstract
Chlorophenols are a class of chemicals commonly used in preservatives, disinfectants, algaecides, herbicides and pesticides. However, there is a growing evidence that these compounds are a threat to human health. This is alarming as many chlorophenols are common pollutants found in the global environment at potentially biohazardous levels. Despite chlorophenols being abundant, widely used and poisonous, we know relatively little about their mechanism of toxicity in eukaryotes. Thus, we performed genome-wide growth screens using Saccharomyces cerevisiae to understand the molecular basis of chlorophenol toxicity. Of ∼4850 single gene knockout strains tested, 393 mutants showed growth sensitivity to treatment with 4-chlorophenol (4-CP),... (More)
Chlorophenols are a class of chemicals commonly used in preservatives, disinfectants, algaecides, herbicides and pesticides. However, there is a growing evidence that these compounds are a threat to human health. This is alarming as many chlorophenols are common pollutants found in the global environment at potentially biohazardous levels. Despite chlorophenols being abundant, widely used and poisonous, we know relatively little about their mechanism of toxicity in eukaryotes. Thus, we performed genome-wide growth screens using Saccharomyces cerevisiae to understand the molecular basis of chlorophenol toxicity. Of ∼4850 single gene knockout strains tested, 393 mutants showed growth sensitivity to treatment with 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) or pentachlorophenol (PCP). Only eight mutants showed growth hypersensitivity to all the three treatments and harboured deletions in genes important for aromatic amino acid biosynthesis (ARO1, ARO7) or mitochondrial protein synthesis and respiration (ATP5, ISA1, RML2, GET2, SLS1, MRPL38). (Less)
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author
; ; and
publishing date
type
Contribution to journal
publication status
published
in
Yeast
volume
28
issue
1
pages
81 - 91
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:78650425704
ISSN
1097-0061
DOI
10.1002/yea.1825
language
English
LU publication?
no
id
cd6940d4-41bc-4b0d-8bf6-da6bc616a000
date added to LUP
2021-12-01 00:05:23
date last changed
2022-04-13 11:48:56
@article{cd6940d4-41bc-4b0d-8bf6-da6bc616a000,
  abstract     = {{Chlorophenols are a class of chemicals commonly used in preservatives, disinfectants, algaecides, herbicides and pesticides. However, there is a growing evidence that these compounds are a threat to human health. This is alarming as many chlorophenols are common pollutants found in the global environment at potentially biohazardous levels. Despite chlorophenols being abundant, widely used and poisonous, we know relatively little about their mechanism of toxicity in eukaryotes. Thus, we performed genome-wide growth screens using Saccharomyces cerevisiae to understand the molecular basis of chlorophenol toxicity. Of ∼4850 single gene knockout strains tested, 393 mutants showed growth sensitivity to treatment with 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) or pentachlorophenol (PCP). Only eight mutants showed growth hypersensitivity to all the three treatments and harboured deletions in genes important for aromatic amino acid biosynthesis (ARO1, ARO7) or mitochondrial protein synthesis and respiration (ATP5, ISA1, RML2, GET2, SLS1, MRPL38).}},
  author       = {{Yadav, Vikas and Shitiz, Kirti and Pandey, Rishi and Yadav, Jyoti}},
  issn         = {{1097-0061}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{81--91}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Yeast}},
  title        = {{Chlorophenol stress affects aromatic amino acid biosynthesis-a genome-wide study}},
  url          = {{http://dx.doi.org/10.1002/yea.1825}},
  doi          = {{10.1002/yea.1825}},
  volume       = {{28}},
  year         = {{2010}},
}