Anti-Inflammatory and Immunoregulatory Activities In Vitro of Coumarins Isolated from the Twigs and Leaves of Wikstroemia indica (L.) C.A. Mey.
(2025) In Natural Product Sciences 31(4). p.284-290- Abstract
Six coumarins were isolated from two bioactive fractions of the 70% ethanol extract from the twigs and leaves of Wikstroemia indica (L.) C.A. Mey. (Thymelaeaceae), namely the ethyl acetate fraction (WIE) and the water fraction (WIW). These compounds were identified as umbelliferone (1), daphnoretin (2), daphnorin (3), adicardin (4), wikstronutin (5), and triumbelletin-7-O-β-D-glucoside (6). Compound 4 is reported for the first time in the genus Wikstroemia, whereas compound 5 is newly identified in this species. All six compounds were evaluated for their effects on Interleukin-1 beta (IL-1β) and IL-10 secretion in Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, among which compounds 1, 2, 5, and 6 showed stronger activity.... (More)
Six coumarins were isolated from two bioactive fractions of the 70% ethanol extract from the twigs and leaves of Wikstroemia indica (L.) C.A. Mey. (Thymelaeaceae), namely the ethyl acetate fraction (WIE) and the water fraction (WIW). These compounds were identified as umbelliferone (1), daphnoretin (2), daphnorin (3), adicardin (4), wikstronutin (5), and triumbelletin-7-O-β-D-glucoside (6). Compound 4 is reported for the first time in the genus Wikstroemia, whereas compound 5 is newly identified in this species. All six compounds were evaluated for their effects on Interleukin-1 beta (IL-1β) and IL-10 secretion in Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, among which compounds 1, 2, 5, and 6 showed stronger activity. These four coumarin derivatives further suppressed the production of Foxp3, Interferon-alpha (IFN-α), IFN-γ, and IL-17 in LPS-stimulated CD4+ T cells. The IC50 values ranged from 18.84 ± 1.16 to 43.45 ± 5.34 μM for Foxp3, 8.96 ± 1.45 to 22.42 ± 4.12 μM for IFN-α, 9.28 ± 1.23 to 16.83 ± 2.15 μM for IFN-γ, and 12.62 ± 2.57 to 22.92 ± 4.41 μM for IL-17. Notably, compound 6 exhibited the strongest immunomodulatory effect. Overall, the findings suggest that coumarins from W. indica possess promising immunomodulatory and anti-inflammatory potential.
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- author
- Nguyen, Van Lieu ; Cao, Van Anh ; Nguyen, Thi Thu ; Tran, Thi Hien LU ; Hoang, Viet Dung and Do, Thi Ha
- organization
- publishing date
- 2025-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CD4 T cells, Coumarin, Cytokines, Immunomodulatory, Wikstroemia indica
- in
- Natural Product Sciences
- volume
- 31
- issue
- 4
- pages
- 7 pages
- publisher
- Korean Society of Pharmacognosy
- external identifiers
-
- scopus:105029864083
- ISSN
- 1226-3907
- DOI
- 10.20307/nps.2025.31.4.284
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025, Korean Society of Pharmacognosy. All rights reserved.
- id
- cd7cb22b-24e8-4d85-8639-0b76ba9ae753
- date added to LUP
- 2026-03-10 12:54:28
- date last changed
- 2026-03-10 12:54:44
@article{cd7cb22b-24e8-4d85-8639-0b76ba9ae753,
abstract = {{<p>Six coumarins were isolated from two bioactive fractions of the 70% ethanol extract from the twigs and leaves of Wikstroemia indica (L.) C.A. Mey. (Thymelaeaceae), namely the ethyl acetate fraction (WIE) and the water fraction (WIW). These compounds were identified as umbelliferone (1), daphnoretin (2), daphnorin (3), adicardin (4), wikstronutin (5), and triumbelletin-7-O-β-D-glucoside (6). Compound 4 is reported for the first time in the genus Wikstroemia, whereas compound 5 is newly identified in this species. All six compounds were evaluated for their effects on Interleukin-1 beta (IL-1β) and IL-10 secretion in Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, among which compounds 1, 2, 5, and 6 showed stronger activity. These four coumarin derivatives further suppressed the production of Foxp3, Interferon-alpha (IFN-α), IFN-γ, and IL-17 in LPS-stimulated CD4<sup>+</sup> T cells. The IC<sub>50</sub> values ranged from 18.84 ± 1.16 to 43.45 ± 5.34 μM for Foxp3, 8.96 ± 1.45 to 22.42 ± 4.12 μM for IFN-α, 9.28 ± 1.23 to 16.83 ± 2.15 μM for IFN-γ, and 12.62 ± 2.57 to 22.92 ± 4.41 μM for IL-17. Notably, compound 6 exhibited the strongest immunomodulatory effect. Overall, the findings suggest that coumarins from W. indica possess promising immunomodulatory and anti-inflammatory potential.</p>}},
author = {{Nguyen, Van Lieu and Cao, Van Anh and Nguyen, Thi Thu and Tran, Thi Hien and Hoang, Viet Dung and Do, Thi Ha}},
issn = {{1226-3907}},
keywords = {{CD4 T cells; Coumarin; Cytokines; Immunomodulatory; Wikstroemia indica}},
language = {{eng}},
number = {{4}},
pages = {{284--290}},
publisher = {{Korean Society of Pharmacognosy}},
series = {{Natural Product Sciences}},
title = {{Anti-Inflammatory and Immunoregulatory Activities In Vitro of Coumarins Isolated from the Twigs and Leaves of Wikstroemia indica (L.) C.A. Mey.}},
url = {{http://dx.doi.org/10.20307/nps.2025.31.4.284}},
doi = {{10.20307/nps.2025.31.4.284}},
volume = {{31}},
year = {{2025}},
}