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Electrochemical Characterization and Bioelectrocatalytic H2O2 Sensing of Non-Symbiotic Hexa-Coordinated Sugar Beet Hemoglobins

Sosna, Maciej ; Leiva-Eriksson, Nélida LU ; Bülow, Leif LU and Ferapontova, Elena E. LU (2020) In ChemElectroChem 7(9). p.2114-2122
Abstract

The biological role of non-symbiotic plant hemoglobins (Hbs) is not well understood. It may involve sensing and signaling of reactive nitrogen and oxygen species–a property that can be used in electrochemical sensing. Here, we electrochemically studied two novel non-symbiotic Beta vulgaris Hbs: BvHb1.2 and BvHb2 expressed in E. coli. At pH 7, we observed close potentials of their Fe2+/3+ hemes, −349 mV for BvHb1.2 and −345/−457 mV vs. Ag/AgCl for the “open” penta-/“closed” hexa-coordinated states of BvHb2. BvHbs bound and bioelectrocatalytically reduced O2 and H2O2 at potentials significantly exceeding their Fe2+/3+ heme potentials. BvHb2, with the onset of... (More)

The biological role of non-symbiotic plant hemoglobins (Hbs) is not well understood. It may involve sensing and signaling of reactive nitrogen and oxygen species–a property that can be used in electrochemical sensing. Here, we electrochemically studied two novel non-symbiotic Beta vulgaris Hbs: BvHb1.2 and BvHb2 expressed in E. coli. At pH 7, we observed close potentials of their Fe2+/3+ hemes, −349 mV for BvHb1.2 and −345/−457 mV vs. Ag/AgCl for the “open” penta-/“closed” hexa-coordinated states of BvHb2. BvHbs bound and bioelectrocatalytically reduced O2 and H2O2 at potentials significantly exceeding their Fe2+/3+ heme potentials. BvHb2, with the onset of H2O2 reduction at 370 mV, enabled O2-interference-free 10 μM H2O2 detection at 0 mV, with a 87 nA μM−1 cm−2 sensitivity comparable to some peroxidases. The results underpin broad electrochemical applications of BvHbs in the electroanalysis of reactive species and in electrochemical biotransformations.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
bioelectrocatalysis, bioelectrochemistry, electrochemical sensing, hexa-coordinated hemoglobins, sugar beet hemoglobins
in
ChemElectroChem
volume
7
issue
9
pages
9 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85084563857
ISSN
2196-0216
DOI
10.1002/celc.202000358
language
English
LU publication?
yes
id
cdf07a5b-9272-4d11-9aa0-c27b8f0c0449
date added to LUP
2020-05-26 13:19:58
date last changed
2020-05-27 01:57:47
@article{cdf07a5b-9272-4d11-9aa0-c27b8f0c0449,
  abstract     = {<p>The biological role of non-symbiotic plant hemoglobins (Hbs) is not well understood. It may involve sensing and signaling of reactive nitrogen and oxygen species–a property that can be used in electrochemical sensing. Here, we electrochemically studied two novel non-symbiotic Beta vulgaris Hbs: BvHb1.2 and BvHb2 expressed in E. coli. At pH 7, we observed close potentials of their Fe<sup>2+/3+</sup> hemes, −349 mV for BvHb1.2 and −345/−457 mV vs. Ag/AgCl for the “open” penta-/“closed” hexa-coordinated states of BvHb2. BvHbs bound and bioelectrocatalytically reduced O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> at potentials significantly exceeding their Fe<sup>2+/3+</sup> heme potentials. BvHb2, with the onset of H<sub>2</sub>O<sub>2</sub> reduction at 370 mV, enabled O<sub>2</sub>-interference-free 10 μM H<sub>2</sub>O<sub>2</sub> detection at 0 mV, with a 87 nA μM<sup>−1</sup> cm<sup>−2</sup> sensitivity comparable to some peroxidases. The results underpin broad electrochemical applications of BvHbs in the electroanalysis of reactive species and in electrochemical biotransformations.</p>},
  author       = {Sosna, Maciej and Leiva-Eriksson, Nélida and Bülow, Leif and Ferapontova, Elena E.},
  issn         = {2196-0216},
  language     = {eng},
  month        = {05},
  number       = {9},
  pages        = {2114--2122},
  publisher    = {Wiley-Blackwell},
  series       = {ChemElectroChem},
  title        = {Electrochemical Characterization and Bioelectrocatalytic H<sub>2</sub>O<sub>2</sub> Sensing of Non-Symbiotic Hexa-Coordinated Sugar Beet Hemoglobins},
  url          = {http://dx.doi.org/10.1002/celc.202000358},
  doi          = {10.1002/celc.202000358},
  volume       = {7},
  year         = {2020},
}