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A third generation glucose biosensor based on cellobiose dehydrogenase immobilized on a glassy carbon electrode decorated with electrodeposited gold nanoparticles : Characterization and application in human saliva

Bollella, Paolo; Gorton, Lo LU ; Ludwig, Roland and Antiochia, Riccarda (2017) In Sensors 17(8).
Abstract

Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19.4 ± 0.6 s−1, with an enhanced theoretical and real enzyme surface coverage (Γtheor and Γreal) of 5287 ± 152 pmol cm−2... (More)

Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19.4 ± 0.6 s−1, with an enhanced theoretical and real enzyme surface coverage (Γtheor and Γreal) of 5287 ± 152 pmol cm−2 and 27 ± 2 pmol cm−2, respectively. The modified electrode was successively used as glucose biosensor exhibiting a detection limit of 6.2 μM, an extended linear range from 0.02 to 30 mM, a sensitivity of 3.1 ± 0.1 μA mM−1 cm−2 (R2 = 0.995), excellent stability and good selectivity. These performances compared favourably with other glucose biosensors reported in the literature. Finally, the biosensor was tested to quantify the glucose content in human saliva samples with successful results in terms of both recovery and correlation with glucose blood levels, allowing further considerations on the development of non-invasive glucose monitoring devices.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cellobiose dehydrogenase, Electrodeposition, Glucose biosensor, Gold nanoparticles, Human saliva
in
Sensors
volume
17
issue
8
publisher
MDPI AG
external identifiers
  • scopus:85028041532
ISSN
1424-8220
DOI
10.3390/s17081912
language
English
LU publication?
yes
id
ce357f34-50c8-4926-9717-486270b876ed
date added to LUP
2017-09-07 08:31:44
date last changed
2017-09-08 03:00:08
@article{ce357f34-50c8-4926-9717-486270b876ed,
  abstract     = {<p>Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (k<sub>s</sub>) of 19.4 ± 0.6 s<sup>−1</sup>, with an enhanced theoretical and real enzyme surface coverage (Γ<sub>theor</sub> and Γ<sub>real</sub>) of 5287 ± 152 pmol cm<sup>−2</sup> and 27 ± 2 pmol cm<sup>−2</sup>, respectively. The modified electrode was successively used as glucose biosensor exhibiting a detection limit of 6.2 μM, an extended linear range from 0.02 to 30 mM, a sensitivity of 3.1 ± 0.1 μA mM<sup>−1</sup> cm<sup>−2</sup> (R<sup>2</sup> = 0.995), excellent stability and good selectivity. These performances compared favourably with other glucose biosensors reported in the literature. Finally, the biosensor was tested to quantify the glucose content in human saliva samples with successful results in terms of both recovery and correlation with glucose blood levels, allowing further considerations on the development of non-invasive glucose monitoring devices.</p>},
  articleno    = {1912},
  author       = {Bollella, Paolo and Gorton, Lo and Ludwig, Roland and Antiochia, Riccarda},
  issn         = {1424-8220},
  keyword      = {Cellobiose dehydrogenase,Electrodeposition,Glucose biosensor,Gold nanoparticles,Human saliva},
  language     = {eng},
  month        = {08},
  number       = {8},
  publisher    = {MDPI AG},
  series       = {Sensors},
  title        = {A third generation glucose biosensor based on cellobiose dehydrogenase immobilized on a glassy carbon electrode decorated with electrodeposited gold nanoparticles : Characterization and application in human saliva},
  url          = {http://dx.doi.org/10.3390/s17081912},
  volume       = {17},
  year         = {2017},
}