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Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus

Barchetta, I.; Ciccarelli, G.; Cimini, F. A.; Ceccarelli, V.; Orho-Melander, M. LU ; Melander, O. LU and Cavallo, M. G. (2018) In Journal of Endocrinological Investigation p.1-5
Abstract

Purpose: Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM. Methods: We recruited a population of 72 subjects (M/F: 39/33; age: 49.5 ± 10.6 years; BMI: 26.5 ± 4.7 kg/m2)... (More)

Purpose: Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM. Methods: We recruited a population of 72 subjects (M/F: 39/33; age: 49.5 ± 10.6 years; BMI: 26.5 ± 4.7 kg/m2) including individuals with T2DM (n = 32) referring to our Diabetes Outpatient Clinics, Sapienza University of Rome, and healthy controls. Study participants underwent metabolic characterization; plasma leptin was measured by MILLIPLEX, Luminex, and proneurotensin (proNT), a stable precursor of NT, by chemiluminometric sandwich immunoassay. Results: Circulating median (25°–75°) leptin levels were 2.75 (1.27–4.93) ng/mL and did not differ between T2DM and non-diabetic subjects. Leptin concentration directly correlated with proNT (r = 0.41; p = 0.015); higher leptin levels were also associated with age, male gender, obesity, higher HOMA-IR, systolic blood pressure and C-reactive protein. Belonging to the highest pro-NT quartile correlated with greater leptin levels independent of age, gender and other confounders (r2 = 0.82, p = 0.02). Conclusions: Circulating leptin is associated with higher proNT levels independent of diabetes, obesity and metabolic syndrome components; besides its effects on central leptin signaling, NT may influence energy balance by modulating circulating leptin concentration likely through a mechanism involving gut fat absorption.

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author
organization
publishing date
type
Contribution to journal
publication status
in press
subject
keywords
Fatty acids, Intestine, Leptin, Neurotensin, Type 2 diabetes mellitus
in
Journal of Endocrinological Investigation
pages
5 pages
external identifiers
  • scopus:85041548894
ISSN
0391-4097
DOI
10.1007/s40618-018-0845-9
language
English
LU publication?
yes
id
ce3f58d8-c4b4-4145-9ddf-f691e47bd558
date added to LUP
2018-03-06 10:42:02
date last changed
2018-05-29 09:43:51
@article{ce3f58d8-c4b4-4145-9ddf-f691e47bd558,
  abstract     = {<p>Purpose: Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM. Methods: We recruited a population of 72 subjects (M/F: 39/33; age: 49.5 ± 10.6 years; BMI: 26.5 ± 4.7 kg/m<sup>2</sup>) including individuals with T2DM (n = 32) referring to our Diabetes Outpatient Clinics, Sapienza University of Rome, and healthy controls. Study participants underwent metabolic characterization; plasma leptin was measured by MILLIPLEX, Luminex, and proneurotensin (proNT), a stable precursor of NT, by chemiluminometric sandwich immunoassay. Results: Circulating median (25°–75°) leptin levels were 2.75 (1.27–4.93) ng/mL and did not differ between T2DM and non-diabetic subjects. Leptin concentration directly correlated with proNT (r = 0.41; p = 0.015); higher leptin levels were also associated with age, male gender, obesity, higher HOMA-IR, systolic blood pressure and C-reactive protein. Belonging to the highest pro-NT quartile correlated with greater leptin levels independent of age, gender and other confounders (r<sup>2</sup> = 0.82, p = 0.02). Conclusions: Circulating leptin is associated with higher proNT levels independent of diabetes, obesity and metabolic syndrome components; besides its effects on central leptin signaling, NT may influence energy balance by modulating circulating leptin concentration likely through a mechanism involving gut fat absorption.</p>},
  author       = {Barchetta, I. and Ciccarelli, G. and Cimini, F. A. and Ceccarelli, V. and Orho-Melander, M. and Melander, O. and Cavallo, M. G.},
  issn         = {0391-4097},
  keyword      = {Fatty acids,Intestine,Leptin,Neurotensin,Type 2 diabetes mellitus},
  language     = {eng},
  month        = {02},
  pages        = {1--5},
  series       = {Journal of Endocrinological Investigation},
  title        = {Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus},
  url          = {http://dx.doi.org/10.1007/s40618-018-0845-9},
  year         = {2018},
}