Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)
(2023) In Methods in molecular biology (Clifton, N.J.) 2652. p.55-77- Abstract
The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.
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https://lup.lub.lu.se/record/ceade5ff-53ce-4a9d-b572-2cb4c36fcdb5
- author
- Sullivan, Heather McDuffie LU ; Krupinska, Ewa LU ; Rasmussen, Anna Andersson LU ; Orozco Rodriguez, Juan Manuel LU and Knecht, Wolfgang LU
- organization
- publishing date
- 2023
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Baculoviridae/genetics, Genetic Vectors, Recombinant Proteins/metabolism
- host publication
- Advanced Methods in Structural Biology
- series title
- Methods in molecular biology (Clifton, N.J.)
- editor
- Sousa, Angela and Passarinha, Luis
- volume
- 2652
- pages
- 55 - 77
- publisher
- Humana Press
- external identifiers
-
- pmid:37093470
- scopus:85153687714
- ISSN
- 1940-6029
- ISBN
- 978-1-0716-3146-1
- 978-1-0716-3147-8
- DOI
- 10.1007/978-1-0716-3147-8_4
- language
- English
- LU publication?
- yes
- additional info
- © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- id
- ceade5ff-53ce-4a9d-b572-2cb4c36fcdb5
- date added to LUP
- 2023-05-09 17:24:07
- date last changed
- 2024-04-19 21:42:17
@inbook{ceade5ff-53ce-4a9d-b572-2cb4c36fcdb5, abstract = {{<p>The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.</p>}}, author = {{Sullivan, Heather McDuffie and Krupinska, Ewa and Rasmussen, Anna Andersson and Orozco Rodriguez, Juan Manuel and Knecht, Wolfgang}}, booktitle = {{Advanced Methods in Structural Biology}}, editor = {{Sousa, Angela and Passarinha, Luis}}, isbn = {{978-1-0716-3146-1}}, issn = {{1940-6029}}, keywords = {{Baculoviridae/genetics; Genetic Vectors; Recombinant Proteins/metabolism}}, language = {{eng}}, pages = {{55--77}}, publisher = {{Humana Press}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)}}, url = {{http://dx.doi.org/10.1007/978-1-0716-3147-8_4}}, doi = {{10.1007/978-1-0716-3147-8_4}}, volume = {{2652}}, year = {{2023}}, }