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Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)

Sullivan, Heather McDuffie LU ; Krupinska, Ewa LU ; Rasmussen, Anna Andersson LU ; Orozco Rodriguez, Juan Manuel LU and Knecht, Wolfgang LU (2023) In Methods in molecular biology (Clifton, N.J.) 2652. p.55-77
Abstract

The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.

Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Baculoviridae/genetics, Genetic Vectors, Recombinant Proteins/metabolism
host publication
Advanced Methods in Structural Biology
series title
Methods in molecular biology (Clifton, N.J.)
editor
Sousa, Angela and Passarinha, Luis
volume
2652
pages
55 - 77
publisher
Humana Press
external identifiers
  • pmid:37093470
  • scopus:85153687714
ISSN
1940-6029
ISBN
978-1-0716-3146-1
978-1-0716-3147-8
DOI
10.1007/978-1-0716-3147-8_4
language
English
LU publication?
yes
additional info
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
id
ceade5ff-53ce-4a9d-b572-2cb4c36fcdb5
date added to LUP
2023-05-09 17:24:07
date last changed
2024-04-19 21:42:17
@inbook{ceade5ff-53ce-4a9d-b572-2cb4c36fcdb5,
  abstract     = {{<p>The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.</p>}},
  author       = {{Sullivan, Heather McDuffie and Krupinska, Ewa and Rasmussen, Anna Andersson and Orozco Rodriguez, Juan Manuel and Knecht, Wolfgang}},
  booktitle    = {{Advanced Methods in Structural Biology}},
  editor       = {{Sousa, Angela and Passarinha, Luis}},
  isbn         = {{978-1-0716-3146-1}},
  issn         = {{1940-6029}},
  keywords     = {{Baculoviridae/genetics; Genetic Vectors; Recombinant Proteins/metabolism}},
  language     = {{eng}},
  pages        = {{55--77}},
  publisher    = {{Humana Press}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3147-8_4}},
  doi          = {{10.1007/978-1-0716-3147-8_4}},
  volume       = {{2652}},
  year         = {{2023}},
}