Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.
(2013) In Acta Crystallographica. Section D: Biological Crystallography 69(Pt 12). p.2524-2533- Abstract
- The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed... (More)
- The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4225145
- author
- Awad, Wael ; Svensson Birkedal, Gabriel LU ; Thunnissen, Marjolein M G M LU ; Mani, Katrin LU and Logan, Derek T
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- 69
- issue
- Pt 12
- pages
- 2524 - 2533
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000328370400025
- pmid:24311593
- scopus:84889768100
- pmid:24311593
- ISSN
- 1399-0047
- DOI
- 10.1107/S0907444913025250
- language
- English
- LU publication?
- yes
- id
- ced2189c-9b32-480f-b97b-0d549d2e3b28 (old id 4225145)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24311593?dopt=Abstract
- date added to LUP
- 2016-04-01 10:16:21
- date last changed
- 2024-08-22 10:45:02
@article{ced2189c-9b32-480f-b97b-0d549d2e3b28, abstract = {{The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.}}, author = {{Awad, Wael and Svensson Birkedal, Gabriel and Thunnissen, Marjolein M G M and Mani, Katrin and Logan, Derek T}}, issn = {{1399-0047}}, language = {{eng}}, number = {{Pt 12}}, pages = {{2524--2533}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Acta Crystallographica. Section D: Biological Crystallography}}, title = {{Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.}}, url = {{http://dx.doi.org/10.1107/S0907444913025250}}, doi = {{10.1107/S0907444913025250}}, volume = {{69}}, year = {{2013}}, }