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Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.

Awad, Wael ; Svensson Birkedal, Gabriel LU ; Thunnissen, Marjolein M G M ; Mani, Katrin LU orcid and Logan, Derek T (2013) In Acta Crystallographica. Section D: Biological Crystallography 69(Pt 12). p.2524-2533
Abstract
The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed... (More)
The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
69
issue
Pt 12
pages
2524 - 2533
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000328370400025
  • pmid:24311593
  • scopus:84889768100
  • pmid:24311593
ISSN
1399-0047
DOI
10.1107/S0907444913025250
language
English
LU publication?
yes
id
ced2189c-9b32-480f-b97b-0d549d2e3b28 (old id 4225145)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24311593?dopt=Abstract
date added to LUP
2016-04-01 10:16:21
date last changed
2023-08-30 22:27:03
@article{ced2189c-9b32-480f-b97b-0d549d2e3b28,
  abstract     = {{The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.}},
  author       = {{Awad, Wael and Svensson Birkedal, Gabriel and Thunnissen, Marjolein M G M and Mani, Katrin and Logan, Derek T}},
  issn         = {{1399-0047}},
  language     = {{eng}},
  number       = {{Pt 12}},
  pages        = {{2524--2533}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Acta Crystallographica. Section D: Biological Crystallography}},
  title        = {{Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.}},
  url          = {{http://dx.doi.org/10.1107/S0907444913025250}},
  doi          = {{10.1107/S0907444913025250}},
  volume       = {{69}},
  year         = {{2013}},
}