Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Design, synthesis, and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors

Purić, Edvin ; Marinović, Marina ; Kojek, Zala ; Kahl-Knutson, Barbro LU ; Leffler, Hakon LU ; Nilsson, Ulf J. LU ; Anderluh, Marko LU and Mravljak, Janez (2025) In Acta Pharmaceutica 75(2). p.273-282
Abstract

In the present work, we describe the design, synthesis, and evaluation of a galectin-8- binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its photophysical properties and its binding affinity for galectin-8N was determined by using FP. We evaluated the probe in a competitive FP assay with three known galectin-8N inhibitors and demonstrated its suitability for high-throughput screening.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Pharmaceutica
volume
75
issue
2
pages
273 - 282
publisher
Hrvatsko Farmaceutsko Drustvo
external identifiers
  • pmid:40305418
  • scopus:105010451026
ISSN
1330-0075
DOI
10.2478/acph-2025-0010
language
English
LU publication?
yes
additional info
© 2025 Edvin Purić et al., published by Sciendo.
id
ced38da4-de58-4e59-9f48-f3dacd95e88e
alternative location
https://sciendo.com/article/10.2478/acph-2025-0010
date added to LUP
2025-05-01 17:03:33
date last changed
2025-11-16 14:25:18
@article{ced38da4-de58-4e59-9f48-f3dacd95e88e,
  abstract     = {{<p>In the present work, we describe the design, synthesis, and evaluation of a galectin-8- binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its photophysical properties and its binding affinity for galectin-8N was determined by using FP. We evaluated the probe in a competitive FP assay with three known galectin-8N inhibitors and demonstrated its suitability for high-throughput screening.</p>}},
  author       = {{Purić, Edvin and Marinović, Marina and Kojek, Zala and Kahl-Knutson, Barbro and Leffler, Hakon and Nilsson, Ulf J. and Anderluh, Marko and Mravljak, Janez}},
  issn         = {{1330-0075}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{2}},
  pages        = {{273--282}},
  publisher    = {{Hrvatsko Farmaceutsko Drustvo}},
  series       = {{Acta Pharmaceutica}},
  title        = {{Design, synthesis, and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors}},
  url          = {{http://dx.doi.org/10.2478/acph-2025-0010}},
  doi          = {{10.2478/acph-2025-0010}},
  volume       = {{75}},
  year         = {{2025}},
}