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Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells

Chou, Song ; Flygare, Johan LU and Lodish, Harvey F. (2013) In Experimental Hematology 41(5). p.479-490
Abstract
We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was... (More)
We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Hematology
volume
41
issue
5
pages
479 - 490
publisher
Elsevier
external identifiers
  • wos:000318666700007
  • scopus:84876864454
ISSN
1873-2399
DOI
10.1016/j.exphem.2013.02.003
language
English
LU publication?
yes
id
cef7c985-b9b7-42d0-a643-df9ead282a8c (old id 3815461)
date added to LUP
2016-04-01 09:55:38
date last changed
2020-01-05 03:41:05
@article{cef7c985-b9b7-42d0-a643-df9ead282a8c,
  abstract     = {We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.},
  author       = {Chou, Song and Flygare, Johan and Lodish, Harvey F.},
  issn         = {1873-2399},
  language     = {eng},
  number       = {5},
  pages        = {479--490},
  publisher    = {Elsevier},
  series       = {Experimental Hematology},
  title        = {Fetal hepatic progenitors support long-term expansion of hematopoietic stem cells},
  url          = {http://dx.doi.org/10.1016/j.exphem.2013.02.003},
  doi          = {10.1016/j.exphem.2013.02.003},
  volume       = {41},
  year         = {2013},
}