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Proof-of-concept method to study uncharacterized methyltransferases using PRDM15

Zhao, Li-Na LU ; Guccione, Ernesto and Kaldis, Philipp LU orcid (2023) In International Journal of Molecular Sciences 24(2). p.1-12
Abstract

The PRDM family of methyltransferases has been implicated in cellular proliferation and differentiation and is deregulated in human diseases, most notably in cancer. PRDMs are related to the SET domain family of methyltransferases; however, from the 19 PRDMs only a few PRDMs with defined enzymatic activities are known. PRDM15 is an uncharacterized transcriptional regulator, with significant structural disorder and lack of defined small-molecule binding pockets. Many aspects of PRDM15 are yet unknown, including its structure, substrates, reaction mechanism, and its methylation profile. Here, we employ a series of computational approaches for an exploratory investigation of its potential substrates and reaction mechanism. Using the... (More)

The PRDM family of methyltransferases has been implicated in cellular proliferation and differentiation and is deregulated in human diseases, most notably in cancer. PRDMs are related to the SET domain family of methyltransferases; however, from the 19 PRDMs only a few PRDMs with defined enzymatic activities are known. PRDM15 is an uncharacterized transcriptional regulator, with significant structural disorder and lack of defined small-molecule binding pockets. Many aspects of PRDM15 are yet unknown, including its structure, substrates, reaction mechanism, and its methylation profile. Here, we employ a series of computational approaches for an exploratory investigation of its potential substrates and reaction mechanism. Using the knowledge of PRDM9 and current knowledge of PRDM15 as basis, we tried to identify genuine substrates of PRDM15. We start from histone-based peptides and learn that the native substrates of PRDM15 may be non-histone proteins. In the future, a combination of sequence-based approaches and signature motif analysis may provide new leads. In summary, our results provide new information about the uncharacterized methyltransferase, PRDM15.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Molecular Sciences
volume
24
issue
2
article number
1327
pages
1 - 12
publisher
MDPI AG
external identifiers
  • scopus:85146780769
  • pmid:36674842
ISSN
1422-0067
DOI
10.3390/ijms24021327
language
English
LU publication?
yes
id
cf039f7f-452e-4ac6-abf1-434705226031
date added to LUP
2023-01-24 10:29:27
date last changed
2024-03-21 17:41:14
@article{cf039f7f-452e-4ac6-abf1-434705226031,
  abstract     = {{<p>The PRDM family of methyltransferases has been implicated in cellular proliferation and differentiation and is deregulated in human diseases, most notably in cancer. PRDMs are related to the SET domain family of methyltransferases; however, from the 19 PRDMs only a few PRDMs with defined enzymatic activities are known. PRDM15 is an uncharacterized transcriptional regulator, with significant structural disorder and lack of defined small-molecule binding pockets. Many aspects of PRDM15 are yet unknown, including its structure, substrates, reaction mechanism, and its methylation profile. Here, we employ a series of computational approaches for an exploratory investigation of its potential substrates and reaction mechanism. Using the knowledge of PRDM9 and current knowledge of PRDM15 as basis, we tried to identify genuine substrates of PRDM15. We start from histone-based peptides and learn that the native substrates of PRDM15 may be non-histone proteins. In the future, a combination of sequence-based approaches and signature motif analysis may provide new leads. In summary, our results provide new information about the uncharacterized methyltransferase, PRDM15.</p>}},
  author       = {{Zhao, Li-Na and Guccione, Ernesto and Kaldis, Philipp}},
  issn         = {{1422-0067}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{1--12}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Proof-of-concept method to study uncharacterized methyltransferases using PRDM15}},
  url          = {{http://dx.doi.org/10.3390/ijms24021327}},
  doi          = {{10.3390/ijms24021327}},
  volume       = {{24}},
  year         = {{2023}},
}