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Effects of formoterol on protein metabolism in myotubes during hyperthermia

Ametller, Elisabet; Busquets, Sílvia; Fuster, Gemma; Figueras, Maria T; Fontes Oliveira, Cibely LU ; Toledo, Míriam; Korzeniewska, Katarzyna; Argilés, Josep M and López-Soriano, Francisco J (2011) In Muscle and Nerve 43(2). p.73-268
Abstract

Proteolysis in skeletal muscle is mainly carried out by the activity of the ubiquitin-dependent proteolytic system. For the study of protein degradation through the ubiquitin-proteasome pathway, we used a model of hyperthermia in murine myotubes. In C2C12 cells, hyperthermia (41°C) induced a significant increase in both the rate of protein synthesis (18%) and degradation (51%). Interestingly, the addition of the β(2) -adrenoceptor agonist formoterol resulted in a significant decrease in protein degradation (21%) without affecting protein synthesis. The decrease in proteolytic rate was associated with decreases in gene expression of the different components of the ubiquitin-dependent proteolytic system. The effects of the β(2) -agonist... (More)

Proteolysis in skeletal muscle is mainly carried out by the activity of the ubiquitin-dependent proteolytic system. For the study of protein degradation through the ubiquitin-proteasome pathway, we used a model of hyperthermia in murine myotubes. In C2C12 cells, hyperthermia (41°C) induced a significant increase in both the rate of protein synthesis (18%) and degradation (51%). Interestingly, the addition of the β(2) -adrenoceptor agonist formoterol resulted in a significant decrease in protein degradation (21%) without affecting protein synthesis. The decrease in proteolytic rate was associated with decreases in gene expression of the different components of the ubiquitin-dependent proteolytic system. The effects of the β(2) -agonist on protein degradation were dependent exclusively on cAMP formation, because inhibition of adenylyl cyclase completely abolished the effects of formoterol on protein degradation. It can be concluded that hyperthermia is a suitable model for studying the anti-proteolytic potential of drugs used in the treatment of muscle wasting.

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publishing date
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Contribution to journal
publication status
published
keywords
Adrenergic beta-2 Receptor Agonists, Analysis of Variance, Animals, Cell Line, Transformed, Cyclic AMP, DDT, Ethanolamines, Formoterol Fumarate, Gene Expression Regulation, Hyperthermia, Induced, Immunosuppressive Agents, Intracellular Fluid, Mice, Muscle Fibers, Skeletal, Myofibrils, Proteasome Endopeptidase Complex, Ubiquitins, Journal Article, Research Support, Non-U.S. Gov't
in
Muscle and Nerve
volume
43
issue
2
pages
6 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:78751654795
ISSN
0148-639X
DOI
10.1002/mus.21852
language
English
LU publication?
no
id
cf675df7-473b-43b7-8b5b-692f6183ade6
date added to LUP
2017-02-28 16:22:44
date last changed
2017-03-12 04:40:51
@article{cf675df7-473b-43b7-8b5b-692f6183ade6,
  abstract     = {<p>Proteolysis in skeletal muscle is mainly carried out by the activity of the ubiquitin-dependent proteolytic system. For the study of protein degradation through the ubiquitin-proteasome pathway, we used a model of hyperthermia in murine myotubes. In C2C12 cells, hyperthermia (41°C) induced a significant increase in both the rate of protein synthesis (18%) and degradation (51%). Interestingly, the addition of the β(2) -adrenoceptor agonist formoterol resulted in a significant decrease in protein degradation (21%) without affecting protein synthesis. The decrease in proteolytic rate was associated with decreases in gene expression of the different components of the ubiquitin-dependent proteolytic system. The effects of the β(2) -agonist on protein degradation were dependent exclusively on cAMP formation, because inhibition of adenylyl cyclase completely abolished the effects of formoterol on protein degradation. It can be concluded that hyperthermia is a suitable model for studying the anti-proteolytic potential of drugs used in the treatment of muscle wasting.</p>},
  author       = {Ametller, Elisabet and Busquets, Sílvia and Fuster, Gemma and Figueras, Maria T and Fontes Oliveira, Cibely and Toledo, Míriam and Korzeniewska, Katarzyna and Argilés, Josep M and López-Soriano, Francisco J},
  issn         = {0148-639X},
  keyword      = {Adrenergic beta-2 Receptor Agonists,Analysis of Variance,Animals,Cell Line, Transformed,Cyclic AMP,DDT,Ethanolamines,Formoterol Fumarate,Gene Expression Regulation,Hyperthermia, Induced,Immunosuppressive Agents,Intracellular Fluid,Mice,Muscle Fibers, Skeletal,Myofibrils,Proteasome Endopeptidase Complex,Ubiquitins,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {2},
  pages        = {73--268},
  publisher    = {John Wiley & Sons},
  series       = {Muscle and Nerve},
  title        = {Effects of formoterol on protein metabolism in myotubes during hyperthermia},
  url          = {http://dx.doi.org/10.1002/mus.21852},
  volume       = {43},
  year         = {2011},
}