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Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications

Moore, Keith J M ; Cahill, Jeremy ; Aidelberg, Guy ; Aronoff, Rachel ; Bektaş, Ali ; Bezdan, Daniela ; Butler, Daniel J ; Chittur, Sridar V ; Codyre, Martin and Federici, Fernan , et al. (2021) In Journal of Biomolecular Techniques 32(3). p.228-275
Abstract

As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the... (More)

As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
COVID-19/diagnosis, COVID-19 Nucleic Acid Testing, Humans, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Pandemics, RNA, Viral, SARS-CoV-2/isolation & purification
in
Journal of Biomolecular Techniques
volume
32
issue
3
pages
228 - 275
publisher
Association of Biomolecular Resource Facilities
external identifiers
  • scopus:85124281562
  • pmid:35136384
ISSN
1524-0215
DOI
10.7171/jbt.21-3203-017
language
English
LU publication?
yes
additional info
© 2021 ABRF.
id
d00d4ef0-a6ee-414c-8e86-7001be9bd113
date added to LUP
2022-02-21 15:34:54
date last changed
2024-04-18 05:52:17
@article{d00d4ef0-a6ee-414c-8e86-7001be9bd113,
  abstract     = {{<p>As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.</p>}},
  author       = {{Moore, Keith J M and Cahill, Jeremy and Aidelberg, Guy and Aronoff, Rachel and Bektaş, Ali and Bezdan, Daniela and Butler, Daniel J and Chittur, Sridar V and Codyre, Martin and Federici, Fernan and Tanner, Nathan A and Tighe, Scott W and True, Randy and Ware, Sarah B and Wyllie, Anne L and Afshin, Evan E and Bendesky, Andres and Chang, Connie B and Dela Rosa, Richard and Elhaik, Eran and Erickson, David and Goldsborough, Andrew S and Grills, George and Hadasch, Kathrin and Hayden, Andrew and Her, Seong-Young and Karl, Julie A and Kim, Chang Hee and Kriegel, Alison J and Kunstman, Thomas and Landau, Zeph and Land, Kevin and Langhorst, Bradley W and Lindner, Ariel B and Mayer, Benjamin E and McLaughlin, Lee A and McLaughlin, Matthew T and Molloy, Jenny and Mozsary, Christopher and Nadler, Jerry L and D'Silva, Melinee and Ng, David and O'Connor, David H and Ongerth, Jerry E and Osuolale, Olayinka and Pinharanda, Ana and Plenker, Dennis and Ranjan, Ravi and Rosbash, Michael and Rotem, Assaf}},
  issn         = {{1524-0215}},
  keywords     = {{COVID-19/diagnosis; COVID-19 Nucleic Acid Testing; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Pandemics; RNA, Viral; SARS-CoV-2/isolation & purification}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{228--275}},
  publisher    = {{Association of Biomolecular Resource Facilities}},
  series       = {{Journal of Biomolecular Techniques}},
  title        = {{Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications}},
  url          = {{http://dx.doi.org/10.7171/jbt.21-3203-017}},
  doi          = {{10.7171/jbt.21-3203-017}},
  volume       = {{32}},
  year         = {{2021}},
}