Rapid Separation of Human Hemoglobin on a Large Scale From Non-clarified Bacterial Cell Homogenates Using Molecularly Imprinted Composite Cryogels
(2021) In Frontiers in Bioengineering and Biotechnology 9.- Abstract
- The production of a macroporous hydrogel column, known as cryogel, has been scaled up (up to 150 mL) in this work for the purification of human hemoglobin from non-clarified bacterial homogenates. Composite cryogels were synthesized in the presence of adult hemoglobin (HbA) to form a molecularly imprinted polymer (MIP)network where the affinity sites for the targeted molecule were placed directly on an acrylamide cryogel by protein imprinting during the cryogelation. The MIP composite cryogel column was first evaluated in a well-defined protein mixture. It showed high selectivity toward HbA in spite of the presence of serum albumin. Also, when examined in complex non-clarified E. coli cell homogenates, the column showed excellent... (More)
- The production of a macroporous hydrogel column, known as cryogel, has been scaled up (up to 150 mL) in this work for the purification of human hemoglobin from non-clarified bacterial homogenates. Composite cryogels were synthesized in the presence of adult hemoglobin (HbA) to form a molecularly imprinted polymer (MIP)network where the affinity sites for the targeted molecule were placed directly on an acrylamide cryogel by protein imprinting during the cryogelation. The MIP composite cryogel column was first evaluated in a well-defined protein mixture. It showed high selectivity toward HbA in spite of the presence of serum albumin. Also, when examined in complex non-clarified E. coli cell homogenates, the column showed excellent chromatographic behavior. The binding capacity of a 50 mL column was thus found to be 0.88 and 1.2 mg/g, from a protein mixture and non-clarified cell homogenate suspension, respectively. The recovery and purification of the 50 mL column for separation of HbA from cell suspension were evaluated to be 79 and 58%, respectively. The MIP affinity cryogel also displayed binding and selectivity toward fetal Hb (HbF) under the same operational conditions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d015d782-43ae-4b8a-9e1a-294f95be2825
- author
- Hajizadeh, Solmaz LU and Kettisen, Karin LU
- contributor
- Bülow, Leif LU and Ye, Lei LU
- organization
- publishing date
- 2021-10-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Frontiers in Bioengineering and Biotechnology
- volume
- 9
- article number
- 671229
- pages
- 11 pages
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:85116978515
- pmid:34660545
- ISSN
- 2296-4185
- DOI
- 10.3389/fbioe.2021.671229
- language
- English
- LU publication?
- yes
- id
- d015d782-43ae-4b8a-9e1a-294f95be2825
- date added to LUP
- 2021-11-09 08:41:57
- date last changed
- 2022-04-27 05:32:45
@article{d015d782-43ae-4b8a-9e1a-294f95be2825, abstract = {{The production of a macroporous hydrogel column, known as cryogel, has been scaled up (up to 150 mL) in this work for the purification of human hemoglobin from non-clarified bacterial homogenates. Composite cryogels were synthesized in the presence of adult hemoglobin (HbA) to form a molecularly imprinted polymer (MIP)network where the affinity sites for the targeted molecule were placed directly on an acrylamide cryogel by protein imprinting during the cryogelation. The MIP composite cryogel column was first evaluated in a well-defined protein mixture. It showed high selectivity toward HbA in spite of the presence of serum albumin. Also, when examined in complex non-clarified <i>E. coli</i> cell homogenates, the column showed excellent chromatographic behavior. The binding capacity of a 50 mL column was thus found to be 0.88 and 1.2 mg/g, from a protein mixture and non-clarified cell homogenate suspension, respectively. The recovery and purification of the 50 mL column for separation of HbA from cell suspension were evaluated to be 79 and 58%, respectively. The MIP affinity cryogel also displayed binding and selectivity toward fetal Hb (HbF) under the same operational conditions.}}, author = {{Hajizadeh, Solmaz and Kettisen, Karin}}, issn = {{2296-4185}}, language = {{eng}}, month = {{10}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Bioengineering and Biotechnology}}, title = {{Rapid Separation of Human Hemoglobin on a Large Scale From Non-clarified Bacterial Cell Homogenates Using Molecularly Imprinted Composite Cryogels}}, url = {{http://dx.doi.org/10.3389/fbioe.2021.671229}}, doi = {{10.3389/fbioe.2021.671229}}, volume = {{9}}, year = {{2021}}, }