Advanced

A comparative study of sex pheromone biosynthesis in two strains of the turnip moth, Agrotis segetum, producing different ratios of sex pheromone components

Wu, Wenqi ; Zhu, Junwei ; Millar, Jocelyn and Löfstedt, Christer LU (1998) In Insect Biochemistry and Molecular Biology 28(11). p.895-900
Abstract

Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation... (More)

Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation between the ratios of pheromone precursors and the ratios of pheromone components. By application of D3-16:COOH, D9-Z11-16:COOH, D9- Z9-14:COOH and D9-Z7-12:COOH to the female pheromone glands, we found that Zimbabwean females produced more labelled D9-Z5-10:OAc than Swedish females. In contrast, in Swedish females, the labelled precursors were mainly converted to D9-Z9-14:OAc and D9-Z7-12:OAc, rather than to D9-Z5-10:OAc. Moreover, the conversion rate of D9-Z5-10:COOH to D9-Z5-10:OAc, was significantly higher in Zimbabwean females than in Swedish females. Our results indicate that differences in chain shortening and/or the preferential reduction of acids with different chain lengths may lead to the production of different ratios. Brain-SOG homogenates from the two populations increased the pheromone production of decapitated females of both populations, but did not change the pheromone ratios of the two populations.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Agrotis segetum, Deuterium-labelled precursors, Pheromone biosynthesis, Ratio regulation
in
Insect Biochemistry and Molecular Biology
volume
28
issue
11
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:0031760661
ISSN
0965-1748
DOI
10.1016/S0965-1748(98)00063-0
project
Evolutionary mechanisms of pheromone divergence in Lepidoptera
language
English
LU publication?
yes
id
d04d2abc-fb43-4c45-b98b-5176bca11d1a
date added to LUP
2020-05-26 15:41:31
date last changed
2020-05-27 01:57:48
@article{d04d2abc-fb43-4c45-b98b-5176bca11d1a,
  abstract     = {<p>Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation between the ratios of pheromone precursors and the ratios of pheromone components. By application of D<sub>3</sub>-16:COOH, D<sub>9</sub>-Z11-16:COOH, D<sub>9</sub>- Z9-14:COOH and D<sub>9</sub>-Z7-12:COOH to the female pheromone glands, we found that Zimbabwean females produced more labelled D<sub>9</sub>-Z5-10:OAc than Swedish females. In contrast, in Swedish females, the labelled precursors were mainly converted to D<sub>9</sub>-Z9-14:OAc and D<sub>9</sub>-Z7-12:OAc, rather than to D<sub>9</sub>-Z5-10:OAc. Moreover, the conversion rate of D<sub>9</sub>-Z5-10:COOH to D<sub>9</sub>-Z5-10:OAc, was significantly higher in Zimbabwean females than in Swedish females. Our results indicate that differences in chain shortening and/or the preferential reduction of acids with different chain lengths may lead to the production of different ratios. Brain-SOG homogenates from the two populations increased the pheromone production of decapitated females of both populations, but did not change the pheromone ratios of the two populations.</p>},
  author       = {Wu, Wenqi and Zhu, Junwei and Millar, Jocelyn and Löfstedt, Christer},
  issn         = {0965-1748},
  language     = {eng},
  month        = {11},
  number       = {11},
  pages        = {895--900},
  publisher    = {Elsevier},
  series       = {Insect Biochemistry and Molecular Biology},
  title        = {A comparative study of sex pheromone biosynthesis in two strains of the turnip moth, Agrotis segetum, producing different ratios of sex pheromone components},
  url          = {http://dx.doi.org/10.1016/S0965-1748(98)00063-0},
  doi          = {10.1016/S0965-1748(98)00063-0},
  volume       = {28},
  year         = {1998},
}