A comparative study of sex pheromone biosynthesis in two strains of the turnip moth, Agrotis segetum, producing different ratios of sex pheromone components
(1998) In Insect Biochemistry and Molecular Biology 28(11). p.895-900- Abstract
Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation... (More)
Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation between the ratios of pheromone precursors and the ratios of pheromone components. By application of D3-16:COOH, D9-Z11-16:COOH, D9- Z9-14:COOH and D9-Z7-12:COOH to the female pheromone glands, we found that Zimbabwean females produced more labelled D9-Z5-10:OAc than Swedish females. In contrast, in Swedish females, the labelled precursors were mainly converted to D9-Z9-14:OAc and D9-Z7-12:OAc, rather than to D9-Z5-10:OAc. Moreover, the conversion rate of D9-Z5-10:COOH to D9-Z5-10:OAc, was significantly higher in Zimbabwean females than in Swedish females. Our results indicate that differences in chain shortening and/or the preferential reduction of acids with different chain lengths may lead to the production of different ratios. Brain-SOG homogenates from the two populations increased the pheromone production of decapitated females of both populations, but did not change the pheromone ratios of the two populations.
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- author
- Wu, Wenqi ; Zhu, Junwei ; Millar, Jocelyn and Löfstedt, Christer LU
- organization
- publishing date
- 1998-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Agrotis segetum, Deuterium-labelled precursors, Pheromone biosynthesis, Ratio regulation
- in
- Insect Biochemistry and Molecular Biology
- volume
- 28
- issue
- 11
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0031760661
- ISSN
- 0965-1748
- DOI
- 10.1016/S0965-1748(98)00063-0
- project
- Evolutionary mechanisms of pheromone divergence in Lepidoptera
- language
- English
- LU publication?
- yes
- id
- d04d2abc-fb43-4c45-b98b-5176bca11d1a
- date added to LUP
- 2020-05-26 15:41:31
- date last changed
- 2022-02-01 06:23:58
@article{d04d2abc-fb43-4c45-b98b-5176bca11d1a,
abstract = {{<p>Among the sex pheromone components of the turnip moth, Agrotis segetum, (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate and (Z)-9-tetradecenyl acetate are biosynthetically derived from palmitic acid by Δ11-desaturation, chain- shortening, reduction and acetylation. Females of a Zimbabwean population produce the three components in a 78:20:2 ratio, while Swedish females produce the three components in a 12:59:29 ratio. We found that the titers of primary pheromone precursors, such as 16:Acyl and Z11-16:Acyl, did not differ significantly between the two populations. However, the amounts of intermediate precursors Z5-10:Acyl, Z7-12:Acyl and Z9-14:Acyl were significantly higher in the Swedish female extracts. There Was no obvious correlation between the ratios of pheromone precursors and the ratios of pheromone components. By application of D<sub>3</sub>-16:COOH, D<sub>9</sub>-Z11-16:COOH, D<sub>9</sub>- Z9-14:COOH and D<sub>9</sub>-Z7-12:COOH to the female pheromone glands, we found that Zimbabwean females produced more labelled D<sub>9</sub>-Z5-10:OAc than Swedish females. In contrast, in Swedish females, the labelled precursors were mainly converted to D<sub>9</sub>-Z9-14:OAc and D<sub>9</sub>-Z7-12:OAc, rather than to D<sub>9</sub>-Z5-10:OAc. Moreover, the conversion rate of D<sub>9</sub>-Z5-10:COOH to D<sub>9</sub>-Z5-10:OAc, was significantly higher in Zimbabwean females than in Swedish females. Our results indicate that differences in chain shortening and/or the preferential reduction of acids with different chain lengths may lead to the production of different ratios. Brain-SOG homogenates from the two populations increased the pheromone production of decapitated females of both populations, but did not change the pheromone ratios of the two populations.</p>}},
author = {{Wu, Wenqi and Zhu, Junwei and Millar, Jocelyn and Löfstedt, Christer}},
issn = {{0965-1748}},
keywords = {{Agrotis segetum; Deuterium-labelled precursors; Pheromone biosynthesis; Ratio regulation}},
language = {{eng}},
month = {{11}},
number = {{11}},
pages = {{895--900}},
publisher = {{Elsevier}},
series = {{Insect Biochemistry and Molecular Biology}},
title = {{A comparative study of sex pheromone biosynthesis in two strains of the turnip moth, Agrotis segetum, producing different ratios of sex pheromone components}},
url = {{http://dx.doi.org/10.1016/S0965-1748(98)00063-0}},
doi = {{10.1016/S0965-1748(98)00063-0}},
volume = {{28}},
year = {{1998}},
}