Occupational exposure to particles and mitochondrial DNA : Relevance for blood pressure
Xu, Yiyi LU ; Li, Huiqi LU ; Hedmer, Maria LU
; Hossain, Mohammad Bakhtiar
; Tinnerberg, Håkan
LU
; Broberg, Karin
LU
and Albin, Maria
LU
(2017)
In Environmental Health: A Global Access Science Source
16(1).
- Abstract
Background: Particle exposure is a risk factor for cardiovascular diseases. Mitochondrial DNA (mtDNA) is a primary target for oxidative stress generated by particle exposure. We aimed to elucidate the effects of occupational exposure to particle-containing welding fumes on different biomarkers of mtDNA function, and in turn, explore if they modify the association between particle exposure and cardiovascular response, measured as blood pressure. Methods: We investigated 101 welders and 127 controls (all non-smoking males) from southern Sweden. Personal sampling of the welders’ exposure to respirable dust was performed during work hours (average sampling time: 6.8 h; range: 2.4-8.6 h) and blood pressure was measured once for each subject.... (More)
Background: Particle exposure is a risk factor for cardiovascular diseases. Mitochondrial DNA (mtDNA) is a primary target for oxidative stress generated by particle exposure. We aimed to elucidate the effects of occupational exposure to particle-containing welding fumes on different biomarkers of mtDNA function, and in turn, explore if they modify the association between particle exposure and cardiovascular response, measured as blood pressure. Methods: We investigated 101 welders and 127 controls (all non-smoking males) from southern Sweden. Personal sampling of the welders’ exposure to respirable dust was performed during work hours (average sampling time: 6.8 h; range: 2.4-8.6 h) and blood pressure was measured once for each subject. We measured relative mtDNA copy number by quantitative PCR and methylation of the mitochondrial regulatory region D-loop and the tRNA encoding gene MT-TF by bisulfite-pyrosequencing. We calculated the relative number of unmethylated D-loop and MT-TF as markers of mtDNA function to explore the modification of mtDNA on the association between particle exposure and blood pressure. General linear models were used for statistical analyses. Results: Welders had higher mtDNA copy number (β = 0.11, p = 0.003) and lower DNA methylation of D-loop (β = −1.4, p = 0.002) and MT-TF (β = −1.5, p = 0.004) than controls. Higher mtDNA copy number was weakly associated with higher personal respirable dust exposure among welders with exposure level above 0.7 mg/m3 (β = 0.037, p = 0.054). MtDNA function modified the effect of welding fumes on blood pressure: welders with low mtDNA function had higher blood pressure than controls, while no such difference was found in the group with high mtDNA function. Conclusion: Increased mtDNA copy number and decreased D-loop and MT-TF methylation were associated with particle-containing welding fumes exposure, indicating exposure-related oxidative stress. The modification of mtDNA function on exposure-associated increase in blood pressure may represent a mitochondria-environment interaction.
(Less)
- author
- Xu, Yiyi
LU
; Li, Huiqi
LU
; Hedmer, Maria
LU
; Hossain, Mohammad Bakhtiar
; Tinnerberg, Håkan
LU
; Broberg, Karin
LU
and Albin, Maria
LU
- organization
- publishing date
- 2017-03-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Blood pressure, Copy number, DNA methylation, Mitochondria, Particle
- in
- Environmental Health: A Global Access Science Source
- volume
- 16
- issue
- 1
- article number
- 22
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:85014839705
- pmid:28274239
- wos:000395975100001
- ISSN
- 1476-069X
- DOI
- 10.1186/s12940-017-0234-4
- language
- English
- LU publication?
- yes
- id
- d05ba3a6-7661-404c-8a0e-d56ec24334ff
- date added to LUP
- 2017-04-10 13:37:55
- date last changed
- 2025-01-07 11:06:03
@article{d05ba3a6-7661-404c-8a0e-d56ec24334ff,
abstract = {{<p>Background: Particle exposure is a risk factor for cardiovascular diseases. Mitochondrial DNA (mtDNA) is a primary target for oxidative stress generated by particle exposure. We aimed to elucidate the effects of occupational exposure to particle-containing welding fumes on different biomarkers of mtDNA function, and in turn, explore if they modify the association between particle exposure and cardiovascular response, measured as blood pressure. Methods: We investigated 101 welders and 127 controls (all non-smoking males) from southern Sweden. Personal sampling of the welders’ exposure to respirable dust was performed during work hours (average sampling time: 6.8 h; range: 2.4-8.6 h) and blood pressure was measured once for each subject. We measured relative mtDNA copy number by quantitative PCR and methylation of the mitochondrial regulatory region D-loop and the tRNA encoding gene MT-TF by bisulfite-pyrosequencing. We calculated the relative number of unmethylated D-loop and MT-TF as markers of mtDNA function to explore the modification of mtDNA on the association between particle exposure and blood pressure. General linear models were used for statistical analyses. Results: Welders had higher mtDNA copy number (β = 0.11, p = 0.003) and lower DNA methylation of D-loop (β = −1.4, p = 0.002) and MT-TF (β = −1.5, p = 0.004) than controls. Higher mtDNA copy number was weakly associated with higher personal respirable dust exposure among welders with exposure level above 0.7 mg/m3 (β = 0.037, p = 0.054). MtDNA function modified the effect of welding fumes on blood pressure: welders with low mtDNA function had higher blood pressure than controls, while no such difference was found in the group with high mtDNA function. Conclusion: Increased mtDNA copy number and decreased D-loop and MT-TF methylation were associated with particle-containing welding fumes exposure, indicating exposure-related oxidative stress. The modification of mtDNA function on exposure-associated increase in blood pressure may represent a mitochondria-environment interaction.</p>}},
author = {{Xu, Yiyi and Li, Huiqi and Hedmer, Maria and Hossain, Mohammad Bakhtiar and Tinnerberg, Håkan and Broberg, Karin and Albin, Maria}},
issn = {{1476-069X}},
keywords = {{Blood pressure; Copy number; DNA methylation; Mitochondria; Particle}},
language = {{eng}},
month = {{03}},
number = {{1}},
publisher = {{BioMed Central (BMC)}},
series = {{Environmental Health: A Global Access Science Source}},
title = {{Occupational exposure to particles and mitochondrial DNA : Relevance for blood pressure}},
url = {{http://dx.doi.org/10.1186/s12940-017-0234-4}},
doi = {{10.1186/s12940-017-0234-4}},
volume = {{16}},
year = {{2017}},
}