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Characterization of airborne ice-nucleation-active bacteria and bacterial fragments

Šantl-Temkiv, Tina ; Sahyoun, Maher LU ; Finster, Kai ; Hartmann, Susan ; Augustin-Bauditz, Stefanie ; Stratmann, Frank ; Wex, Heike ; Clauss, Tina ; Nielsen, Niels Woetmann and Sørensen, Jens Havskov , et al. (2015) In Atmospheric Environment 109. p.105-117
Abstract

Some bacteria have the unique capacity of synthesising ice-nucleation-active (INA) proteins and exposing them at their outer membrane surface. As INA bacteria enter the atmosphere, they may impact the formation of clouds and precipitation. We studied members of airborne bacterial communities for their capacity to catalyse ice formation and we report on the excretion of INA proteins by airborne Pseudomonas sp. We also observed for the first time that INA biological fragments <220nm were present in precipitation samples (199 and 482 INA fragments per L of precipitation), which confirms the presence of submicron INA biological fragments in the atmosphere. During 14 precipitation events, strains affiliated with the genus Pseudomonas,... (More)

Some bacteria have the unique capacity of synthesising ice-nucleation-active (INA) proteins and exposing them at their outer membrane surface. As INA bacteria enter the atmosphere, they may impact the formation of clouds and precipitation. We studied members of airborne bacterial communities for their capacity to catalyse ice formation and we report on the excretion of INA proteins by airborne Pseudomonas sp. We also observed for the first time that INA biological fragments <220nm were present in precipitation samples (199 and 482 INA fragments per L of precipitation), which confirms the presence of submicron INA biological fragments in the atmosphere. During 14 precipitation events, strains affiliated with the genus Pseudomonas, which are known to carry ina genes, were dominant. A screening for INA properties revealed that ~12% of the cultivable bacteria caused ice formation at ≤-7°C. They had likely been emitted to the atmosphere from terrestrial surfaces, e.g. by convective transport. We tested the ability of isolated INA strains to produce outer membrane vesicles and found that two isolates could do so. However, only very few INA vesicles were released per INA cell. Thus, the source of the submicron INA proteinaceous particles that we detected in the atmosphere remains to be elucidated.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aeromicrobiology, Airborne bacteria, Bioprecipitation, Ice nucleation, INA bacteria, Outer membrane vesicles
in
Atmospheric Environment
volume
109
pages
13 pages
publisher
Elsevier
external identifiers
  • scopus:84924405751
ISSN
1352-2310
DOI
10.1016/j.atmosenv.2015.02.060
language
English
LU publication?
no
id
d076848b-1238-446f-b868-57a631c3784a
date added to LUP
2019-12-17 12:11:38
date last changed
2022-04-02 23:41:24
@article{d076848b-1238-446f-b868-57a631c3784a,
  abstract     = {{<p>Some bacteria have the unique capacity of synthesising ice-nucleation-active (INA) proteins and exposing them at their outer membrane surface. As INA bacteria enter the atmosphere, they may impact the formation of clouds and precipitation. We studied members of airborne bacterial communities for their capacity to catalyse ice formation and we report on the excretion of INA proteins by airborne Pseudomonas sp. We also observed for the first time that INA biological fragments &lt;220nm were present in precipitation samples (199 and 482 INA fragments per L of precipitation), which confirms the presence of submicron INA biological fragments in the atmosphere. During 14 precipitation events, strains affiliated with the genus Pseudomonas, which are known to carry ina genes, were dominant. A screening for INA properties revealed that ~12% of the cultivable bacteria caused ice formation at ≤-7°C. They had likely been emitted to the atmosphere from terrestrial surfaces, e.g. by convective transport. We tested the ability of isolated INA strains to produce outer membrane vesicles and found that two isolates could do so. However, only very few INA vesicles were released per INA cell. Thus, the source of the submicron INA proteinaceous particles that we detected in the atmosphere remains to be elucidated.</p>}},
  author       = {{Šantl-Temkiv, Tina and Sahyoun, Maher and Finster, Kai and Hartmann, Susan and Augustin-Bauditz, Stefanie and Stratmann, Frank and Wex, Heike and Clauss, Tina and Nielsen, Niels Woetmann and Sørensen, Jens Havskov and Korsholm, Ulrik Smith and Wick, Lukas Y. and Karlson, Ulrich Gosewinkel}},
  issn         = {{1352-2310}},
  keywords     = {{Aeromicrobiology; Airborne bacteria; Bioprecipitation; Ice nucleation; INA bacteria; Outer membrane vesicles}},
  language     = {{eng}},
  month        = {{05}},
  pages        = {{105--117}},
  publisher    = {{Elsevier}},
  series       = {{Atmospheric Environment}},
  title        = {{Characterization of airborne ice-nucleation-active bacteria and bacterial fragments}},
  url          = {{http://dx.doi.org/10.1016/j.atmosenv.2015.02.060}},
  doi          = {{10.1016/j.atmosenv.2015.02.060}},
  volume       = {{109}},
  year         = {{2015}},
}