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Neutral microprotein, a novel human plasma and urinary protein associated with a yellow-brown chromophore. Isolation from protein HC preparations and partial characterization

López-Otin, C ; Grubb, A O LU orcid and Méndez, E (1983) In Biochemical and Biophysical Research Communications 117(1). p.9-202
Abstract

An apparently novel human plasma and urinary protein of low molecular weight was isolated from several highly purified preparations of protein HC by gel chromatography and high voltage electrophoresis with a yield of about 8 mg/g. The protein has a molecular weight of about 20,000, neutral electrophoretic mobility at pH 6.5 and a high content of half-cystine. It is associated with a yellow-brown chromophore like protein HC and could be demonstrated in all investigated preparations of isolated human, rabbit and guinea-pig protein HC and alpha 1-microglobulin.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins/blood, Amino Acids/analysis, Chromatography, Gel, Chromatography, Ion Exchange, Humans
in
Biochemical and Biophysical Research Communications
volume
117
issue
1
pages
9 - 202
publisher
Elsevier
external identifiers
  • scopus:0021116221
  • pmid:6197970
ISSN
0006-291X
DOI
10.1016/0006-291x(83)91561-9
language
English
LU publication?
yes
id
d0c4bf72-7e72-41e4-91ba-489e53104737
date added to LUP
2021-10-25 11:50:19
date last changed
2024-01-12 02:54:52
@article{d0c4bf72-7e72-41e4-91ba-489e53104737,
  abstract     = {{<p>An apparently novel human plasma and urinary protein of low molecular weight was isolated from several highly purified preparations of protein HC by gel chromatography and high voltage electrophoresis with a yield of about 8 mg/g. The protein has a molecular weight of about 20,000, neutral electrophoretic mobility at pH 6.5 and a high content of half-cystine. It is associated with a yellow-brown chromophore like protein HC and could be demonstrated in all investigated preparations of isolated human, rabbit and guinea-pig protein HC and alpha 1-microglobulin.</p>}},
  author       = {{López-Otin, C and Grubb, A O and Méndez, E}},
  issn         = {{0006-291X}},
  keywords     = {{Alpha-Globulins/blood; Amino Acids/analysis; Chromatography, Gel; Chromatography, Ion Exchange; Humans}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{9--202}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Neutral microprotein, a novel human plasma and urinary protein associated with a yellow-brown chromophore. Isolation from protein HC preparations and partial characterization}},
  url          = {{http://dx.doi.org/10.1016/0006-291x(83)91561-9}},
  doi          = {{10.1016/0006-291x(83)91561-9}},
  volume       = {{117}},
  year         = {{1983}},
}