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Dose efficient compton X-ray microscopy

Villanueva-Perez, P. LU ; Bajt, S. and Chapman, H. N. (2018) In Optica 5(4). p.450-457
Abstract

X-ray imaging techniques have proven invaluable to study biological systems at high resolution due to the penetration power and short wavelength of this radiation. In practice, the resolution and sensitivity of current X-ray imaging techniques are not limited by the performance of optics or image-recovery methods but by radiation damage. We propose the use of Compton (inelastic) X-ray scattering for high-resolution cellular imaging and provide a study of a scanning microscope geometry that requires a dose to achieve a given resolution that is three orders of magnitude lower than for coherent (elastic) scattering. We find that the dose per imaging signal is minimized at a photon energy of 64 keV. This corresponds to a short enough... (More)

X-ray imaging techniques have proven invaluable to study biological systems at high resolution due to the penetration power and short wavelength of this radiation. In practice, the resolution and sensitivity of current X-ray imaging techniques are not limited by the performance of optics or image-recovery methods but by radiation damage. We propose the use of Compton (inelastic) X-ray scattering for high-resolution cellular imaging and provide a study of a scanning microscope geometry that requires a dose to achieve a given resolution that is three orders of magnitude lower than for coherent (elastic) scattering. We find that the dose per imaging signal is minimized at a photon energy of 64 keV. This corresponds to a short enough wavelength (0.02 nm) to provide nanometer transverse resolution and micrometer depth of field for tomographic imaging of whole cells. The microscope could be implemented at future high-energy and high-brightness synchrotron-radiation facilities to provide images of unsectioned and unlabeled cells in their native conditions at enough detail to bridge the techniques of super-resolution optical microscopy and cryo-electron microscopy.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Optica
volume
5
issue
4
pages
8 pages
publisher
Optical Society of America
external identifiers
  • scopus:85045962060
ISSN
2334-2536
DOI
10.1364/OPTICA.5.000450
language
English
LU publication?
no
id
d0f1b40c-bf6a-4381-8071-0a873c49de5f
date added to LUP
2019-03-29 16:47:09
date last changed
2019-06-11 04:02:32
@article{d0f1b40c-bf6a-4381-8071-0a873c49de5f,
  abstract     = {<p>X-ray imaging techniques have proven invaluable to study biological systems at high resolution due to the penetration power and short wavelength of this radiation. In practice, the resolution and sensitivity of current X-ray imaging techniques are not limited by the performance of optics or image-recovery methods but by radiation damage. We propose the use of Compton (inelastic) X-ray scattering for high-resolution cellular imaging and provide a study of a scanning microscope geometry that requires a dose to achieve a given resolution that is three orders of magnitude lower than for coherent (elastic) scattering. We find that the dose per imaging signal is minimized at a photon energy of 64 keV. This corresponds to a short enough wavelength (0.02 nm) to provide nanometer transverse resolution and micrometer depth of field for tomographic imaging of whole cells. The microscope could be implemented at future high-energy and high-brightness synchrotron-radiation facilities to provide images of unsectioned and unlabeled cells in their native conditions at enough detail to bridge the techniques of super-resolution optical microscopy and cryo-electron microscopy.</p>},
  author       = {Villanueva-Perez, P. and Bajt, S. and Chapman, H. N.},
  issn         = {2334-2536},
  language     = {eng},
  month        = {04},
  number       = {4},
  pages        = {450--457},
  publisher    = {Optical Society of America},
  series       = {Optica},
  title        = {Dose efficient compton X-ray microscopy},
  url          = {http://dx.doi.org/10.1364/OPTICA.5.000450},
  volume       = {5},
  year         = {2018},
}