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In vitro lymphocyte recognition of islet cells following in vivo priming with allogeneic murine pancreatic islets

Scott, J. ; MacKay, P. G. and Lernmark, A. LU orcid (1984) In Acta Endocrinologica 105(1). p.87-92
Abstract

Lymphocytes from patients with insulin-dependent diabetes have been shown to be sensitized to pancreatic tissue antigens. Mice immunized with homologous pancreatic islets have been found to develop glucose intolerance and insulitis. Since lymphocytes may be involved in diabetogenesis, we wished to determine if lymph node cells from islet-immunized mice can recognize and respond to islet cells in vitro. A.TL female mice were immunized with an emulsion of BALB/c islet homogenate and complete Freund's adjuvant (CFA); sham-treated A.TL mice were injected with adjuvant and water. Mice were sacrificed 7-8 days later and the draining lymph nodes were removed. The lymph node cells were co-cultured with freshly prepared irradiated BALB/c islet... (More)

Lymphocytes from patients with insulin-dependent diabetes have been shown to be sensitized to pancreatic tissue antigens. Mice immunized with homologous pancreatic islets have been found to develop glucose intolerance and insulitis. Since lymphocytes may be involved in diabetogenesis, we wished to determine if lymph node cells from islet-immunized mice can recognize and respond to islet cells in vitro. A.TL female mice were immunized with an emulsion of BALB/c islet homogenate and complete Freund's adjuvant (CFA); sham-treated A.TL mice were injected with adjuvant and water. Mice were sacrificed 7-8 days later and the draining lymph nodes were removed. The lymph node cells were co-cultured with freshly prepared irradiated BALB/c islet cell, which served as stimulator cells. The co-cultures were incubated for 24-26 h at 37° C, followed by a 16 h [3H]thymidine (TdR) pulse. A significant proliferation of lymph node cells from islet-primed mice was induced during the in vitro stimulation with irradiated islet cells when compared with lymph node cells from sham-treated mice (P < 0.001). The response may be islet-cell-specific, since irradiated lymph node cells from BALB/c mice failed to elicit a proliferative response under the same culture conditions (P > 0.80). It is suggested that pancreatic islet cells have an ability to stimulate proliferation of lymphocytes which recognize islet-cell-specific antigens. This lymphocyte recognition phenomenon may be relevant to our understanding of autoimmune mechanisms in insulin-dependent diabetes mellitus.

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author
; and
publishing date
type
Contribution to journal
publication status
published
in
Acta Endocrinologica
volume
105
issue
1
pages
6 pages
publisher
Society of the European Journal of Endocrinology
external identifiers
  • scopus:0021344130
  • pmid:6229965
ISSN
0001-5598
language
English
LU publication?
no
id
d0f5c0b2-6b99-4778-bb97-1419dbe15748
date added to LUP
2019-09-16 12:40:45
date last changed
2024-03-13 08:24:54
@article{d0f5c0b2-6b99-4778-bb97-1419dbe15748,
  abstract     = {{<p>Lymphocytes from patients with insulin-dependent diabetes have been shown to be sensitized to pancreatic tissue antigens. Mice immunized with homologous pancreatic islets have been found to develop glucose intolerance and insulitis. Since lymphocytes may be involved in diabetogenesis, we wished to determine if lymph node cells from islet-immunized mice can recognize and respond to islet cells in vitro. A.TL female mice were immunized with an emulsion of BALB/c islet homogenate and complete Freund's adjuvant (CFA); sham-treated A.TL mice were injected with adjuvant and water. Mice were sacrificed 7-8 days later and the draining lymph nodes were removed. The lymph node cells were co-cultured with freshly prepared irradiated BALB/c islet cell, which served as stimulator cells. The co-cultures were incubated for 24-26 h at 37° C, followed by a 16 h [<sup>3</sup>H]thymidine (TdR) pulse. A significant proliferation of lymph node cells from islet-primed mice was induced during the in vitro stimulation with irradiated islet cells when compared with lymph node cells from sham-treated mice (P &lt; 0.001). The response may be islet-cell-specific, since irradiated lymph node cells from BALB/c mice failed to elicit a proliferative response under the same culture conditions (P &gt; 0.80). It is suggested that pancreatic islet cells have an ability to stimulate proliferation of lymphocytes which recognize islet-cell-specific antigens. This lymphocyte recognition phenomenon may be relevant to our understanding of autoimmune mechanisms in insulin-dependent diabetes mellitus.</p>}},
  author       = {{Scott, J. and MacKay, P. G. and Lernmark, A.}},
  issn         = {{0001-5598}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{87--92}},
  publisher    = {{Society of the European Journal of Endocrinology}},
  series       = {{Acta Endocrinologica}},
  title        = {{In vitro lymphocyte recognition of islet cells following in vivo priming with allogeneic murine pancreatic islets}},
  volume       = {{105}},
  year         = {{1984}},
}