Progressive improvement in wound healing with increased therapy in haemophilia B mice
(2013) In Haemophilia 19(6). p.926-932- Abstract
- Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed... (More)
- Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed shortened time to healing relative to untreated animals. Haematoma formation was prevented by treatment and bleeding into the wounds, measured by iron scores, was reduced by treatment. In addition, there was a progressive improvement in healing with 7days of treatment more effective than 5days which was more effective than 3days. Replacement therapy with FIX had slightly shorter healing times than bypassing therapy with FVIIa. HB mice treated with FIX had slightly smaller wound area than untreated animals; by contrast, FVIIa-treated animals had much smaller wound areas that were close to the wound areas seen in wild-type animals. The data suggest that sustained therapy is required for normal wound healing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4204511
- author
- Monroe, D. M. ; Hoffman, M. ; Roberts, H. R. and Hedner, Ulla LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bleeding, factor IX, factor VIIa, haemophilia, mouse models, wound, healing
- in
- Haemophilia
- volume
- 19
- issue
- 6
- pages
- 926 - 932
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000326105100042
- scopus:84886641038
- pmid:23879625
- ISSN
- 1351-8216
- DOI
- 10.1111/hae.12220
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200)
- id
- d104bf08-74af-43a6-9b33-ed6b37588237 (old id 4204511)
- date added to LUP
- 2016-04-01 09:51:33
- date last changed
- 2022-06-15 03:43:30
@article{d104bf08-74af-43a6-9b33-ed6b37588237,
abstract = {{Previous work has shown that normalized haemostasis only at the time of an injury is not sufficient to promote optimal wound healing in haemophilia B (HB) mice. However, the duration of treatment required for optimal healing has not been established. The goal of these studies was to determine the effect of different durations of replacement or bypassing therapy [factor IX(FIX) or factor VIIa (FVIIa)] on wound healing parameters in a mouse model of HB. A dermal wound was placed on the back of HB mice. Animals were either untreated or pretreated and then subsequently treated for 3days, 5days, or 7days with FIX or FVIIa. Wound area, time to wound healing, haematoma formation and iron deposition were measured. All treated animals showed shortened time to healing relative to untreated animals. Haematoma formation was prevented by treatment and bleeding into the wounds, measured by iron scores, was reduced by treatment. In addition, there was a progressive improvement in healing with 7days of treatment more effective than 5days which was more effective than 3days. Replacement therapy with FIX had slightly shorter healing times than bypassing therapy with FVIIa. HB mice treated with FIX had slightly smaller wound area than untreated animals; by contrast, FVIIa-treated animals had much smaller wound areas that were close to the wound areas seen in wild-type animals. The data suggest that sustained therapy is required for normal wound healing.}},
author = {{Monroe, D. M. and Hoffman, M. and Roberts, H. R. and Hedner, Ulla}},
issn = {{1351-8216}},
keywords = {{bleeding; factor IX; factor VIIa; haemophilia; mouse models; wound; healing}},
language = {{eng}},
number = {{6}},
pages = {{926--932}},
publisher = {{Wiley-Blackwell}},
series = {{Haemophilia}},
title = {{Progressive improvement in wound healing with increased therapy in haemophilia B mice}},
url = {{http://dx.doi.org/10.1111/hae.12220}},
doi = {{10.1111/hae.12220}},
volume = {{19}},
year = {{2013}},
}