Characterization of nitrotyrosine as a biomarker for arthritis and joint injury
Misko, T. P. ; Radabaugh, M. R. ; Highkin, M. ; Abrams, M. ; Friese, O. ; Gallavan, R. ; Bramson, C. ; Le Graverand, M. P. Hellio ; Lohmander, Stefan LU
and Roman, D.
(2013)
In Osteoarthritis and Cartilage
21(1).
p.151-156
- Abstract
- Objectives: To characterize the utility of nitrotyrosine (NT) as a biomarker for arthritis and joint injury. Design: Synovial fluid, plasma, and urine from patients diagnosed with osteoarthritis (OA), rheumatoid arthritis (RA), anterior cruciate ligament (ACL) injury, meniscus injury and pseudogout, and knee-healthy volunteers were analyzed for concentrations of NT, nitrate and nitrite (NOx), matrix metalloproteinase (MMP)-3, MMP-1, MMP-9, more than 40 chemokines and cytokines. Results: In OA, plasma and synovial fluid NT were increased versus healthy volunteers. Synovial fluid to plasma NT ratios were elevated in OA patients. Synovial fluid from patients with ACL and meniscus injury and pseudogout had increased levels of NT (P <... (More)
- Objectives: To characterize the utility of nitrotyrosine (NT) as a biomarker for arthritis and joint injury. Design: Synovial fluid, plasma, and urine from patients diagnosed with osteoarthritis (OA), rheumatoid arthritis (RA), anterior cruciate ligament (ACL) injury, meniscus injury and pseudogout, and knee-healthy volunteers were analyzed for concentrations of NT, nitrate and nitrite (NOx), matrix metalloproteinase (MMP)-3, MMP-1, MMP-9, more than 40 chemokines and cytokines. Results: In OA, plasma and synovial fluid NT were increased versus healthy volunteers. Synovial fluid to plasma NT ratios were elevated in OA patients. Synovial fluid from patients with ACL and meniscus injury and pseudogout had increased levels of NT (P < 0.001). In these samples, NT levels significantly correlated with ARGS-aggrecan neoepitope generated by aggrecanase cleavage of aggrecan (P <= 0.001), cross-linked C-telopeptides of type II collagen (P < 0.001), MMP-1 (P = 0.008), and MMP-3 (P <= 0.001). In RA, plasma NT decreased following 6 months of anti-tumor necrosis factor (TNF) treatment. For every 1.1% change in log(10) NT, there was a 1.0% change in the log(10) disease activity scores (DAS28-3 CRP). Both predicted and observed DAS28-3 CRP showed a robust linear relationship with NT. RA plasma NT positively correlated with CRP, MMP-3 and interferon gamma-induced protein 10. Conclusions: NT may serve as a useful biomarker for arthritis and joint injury. In RA, NT is highly correlated with several biomarkers and clinical correlates of disease activity and responds to anti-TNF therapy. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3590089
- author
- Misko, T. P.
; Radabaugh, M. R.
; Highkin, M.
; Abrams, M.
; Friese, O.
; Gallavan, R.
; Bramson, C.
; Le Graverand, M. P. Hellio
; Lohmander, Stefan
LU
and Roman, D.
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nitric oxide, Nitrotyrosine, Biomarkers, Arthritis, Joint injury
- in
- Osteoarthritis and Cartilage
- volume
- 21
- issue
- 1
- pages
- 151 - 156
- publisher
- Elsevier
- external identifiers
-
- wos:000314480200020
- scopus:84871921861
- pmid:23025928
- ISSN
- 1063-4584
- DOI
- 10.1016/j.joca.2012.09.005
- language
- English
- LU publication?
- yes
- id
- d115b261-4fd0-4c11-a42d-f1b02286cd7b (old id 3590089)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23025928
- date added to LUP
- 2016-04-01 09:55:24
- date last changed
- 2023-01-25 02:44:38
@article{d115b261-4fd0-4c11-a42d-f1b02286cd7b,
abstract = {{Objectives: To characterize the utility of nitrotyrosine (NT) as a biomarker for arthritis and joint injury. Design: Synovial fluid, plasma, and urine from patients diagnosed with osteoarthritis (OA), rheumatoid arthritis (RA), anterior cruciate ligament (ACL) injury, meniscus injury and pseudogout, and knee-healthy volunteers were analyzed for concentrations of NT, nitrate and nitrite (NOx), matrix metalloproteinase (MMP)-3, MMP-1, MMP-9, more than 40 chemokines and cytokines. Results: In OA, plasma and synovial fluid NT were increased versus healthy volunteers. Synovial fluid to plasma NT ratios were elevated in OA patients. Synovial fluid from patients with ACL and meniscus injury and pseudogout had increased levels of NT (P < 0.001). In these samples, NT levels significantly correlated with ARGS-aggrecan neoepitope generated by aggrecanase cleavage of aggrecan (P <= 0.001), cross-linked C-telopeptides of type II collagen (P < 0.001), MMP-1 (P = 0.008), and MMP-3 (P <= 0.001). In RA, plasma NT decreased following 6 months of anti-tumor necrosis factor (TNF) treatment. For every 1.1% change in log(10) NT, there was a 1.0% change in the log(10) disease activity scores (DAS28-3 CRP). Both predicted and observed DAS28-3 CRP showed a robust linear relationship with NT. RA plasma NT positively correlated with CRP, MMP-3 and interferon gamma-induced protein 10. Conclusions: NT may serve as a useful biomarker for arthritis and joint injury. In RA, NT is highly correlated with several biomarkers and clinical correlates of disease activity and responds to anti-TNF therapy. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.}},
author = {{Misko, T. P. and Radabaugh, M. R. and Highkin, M. and Abrams, M. and Friese, O. and Gallavan, R. and Bramson, C. and Le Graverand, M. P. Hellio and Lohmander, Stefan and Roman, D.}},
issn = {{1063-4584}},
keywords = {{Nitric oxide; Nitrotyrosine; Biomarkers; Arthritis; Joint injury}},
language = {{eng}},
number = {{1}},
pages = {{151--156}},
publisher = {{Elsevier}},
series = {{Osteoarthritis and Cartilage}},
title = {{Characterization of nitrotyrosine as a biomarker for arthritis and joint injury}},
url = {{https://lup.lub.lu.se/search/files/1390549/3814950.pdf}},
doi = {{10.1016/j.joca.2012.09.005}},
volume = {{21}},
year = {{2013}},
}