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Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein

Callesen, Katrine T. ; Mogren, Sofia LU ; Berlin, Frida LU ; Andersson, Cecilia LU ; Schmidt, Susanne ; Klitfod, Lotte ; Esteban, Vanesa ; Poulsen, Lars K. and Jensen, Bettina M. (2022) In Biomedicines 10(5).
Abstract

Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64%... (More)

Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64% MCT). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcɛRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
C3a, C5a, histamine, mast cells, MRGPRX2, PAF, tryptase, vascular system
in
Biomedicines
volume
10
issue
5
article number
1062
publisher
MDPI AG
external identifiers
  • pmid:35625799
  • scopus:85130015505
ISSN
2227-9059
DOI
10.3390/biomedicines10051062
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
id
d12e8fbf-efaa-41c2-adf3-c26d058924f7
date added to LUP
2022-08-19 13:59:23
date last changed
2023-03-16 18:22:30
@article{d12e8fbf-efaa-41c2-adf3-c26d058924f7,
  abstract     = {{<p>Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64% MCT). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcɛRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.</p>}},
  author       = {{Callesen, Katrine T. and Mogren, Sofia and Berlin, Frida and Andersson, Cecilia and Schmidt, Susanne and Klitfod, Lotte and Esteban, Vanesa and Poulsen, Lars K. and Jensen, Bettina M.}},
  issn         = {{2227-9059}},
  keywords     = {{C3a; C5a; histamine; mast cells; MRGPRX2; PAF; tryptase; vascular system}},
  language     = {{eng}},
  number       = {{5}},
  publisher    = {{MDPI AG}},
  series       = {{Biomedicines}},
  title        = {{Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein}},
  url          = {{http://dx.doi.org/10.3390/biomedicines10051062}},
  doi          = {{10.3390/biomedicines10051062}},
  volume       = {{10}},
  year         = {{2022}},
}