Dual-band fluorosensor for discriminating non-eating from algae-eating zooplankton in aquatic environments
(2020) In OSA Continuum 3(7). p.1730-1738- Abstract
We present a fluorosensor for the detection of laser-induced autofluorescence of zooplankton in marine environments. The sensor uses an inexpensive 410 nm laser diode as excitation source and simultaneously measures two fluorescence bands, 500-550 nm and 675-725 nm, using two identical 16-bit linear array detectors. We show continuous measurements at 200 Hz of zooplankton swimming through a water volume illuminated by the 410 nm laser. The sensor can distinguish salmon lice (Lepeophtheirus salmonis) larvae from an algae-eating reference species (Acartia tonsa) with a sensitivity of up to 99%. The system successfully differentiates the two species using mixed-species cultures at different ratios. This work shows the potential of... (More)
We present a fluorosensor for the detection of laser-induced autofluorescence of zooplankton in marine environments. The sensor uses an inexpensive 410 nm laser diode as excitation source and simultaneously measures two fluorescence bands, 500-550 nm and 675-725 nm, using two identical 16-bit linear array detectors. We show continuous measurements at 200 Hz of zooplankton swimming through a water volume illuminated by the 410 nm laser. The sensor can distinguish salmon lice (Lepeophtheirus salmonis) larvae from an algae-eating reference species (Acartia tonsa) with a sensitivity of up to 99%. The system successfully differentiates the two species using mixed-species cultures at different ratios. This work shows the potential of fluorescent pest monitoring in the salmon farming industry and paves the way for single-ended aquatic lidars.
(Less)
- author
- Nielsen, J. H. ; Pedersen, C. ; Kiørboe, T. ; Nikolajsen, T. ; Brydegaard, M. LU and Rodrigo, P. J.
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- OSA Continuum
- volume
- 3
- issue
- 7
- pages
- 9 pages
- publisher
- Optical Society of America
- external identifiers
-
- scopus:85105689649
- ISSN
- 2578-7519
- DOI
- 10.1364/OSAC.389089
- language
- English
- LU publication?
- yes
- id
- d17da63a-dd0a-4d7f-9737-828002a6e531
- date added to LUP
- 2021-05-28 09:33:30
- date last changed
- 2022-04-19 06:31:28
@article{d17da63a-dd0a-4d7f-9737-828002a6e531,
abstract = {{<p>We present a fluorosensor for the detection of laser-induced autofluorescence of zooplankton in marine environments. The sensor uses an inexpensive 410 nm laser diode as excitation source and simultaneously measures two fluorescence bands, 500-550 nm and 675-725 nm, using two identical 16-bit linear array detectors. We show continuous measurements at 200 Hz of zooplankton swimming through a water volume illuminated by the 410 nm laser. The sensor can distinguish salmon lice (Lepeophtheirus salmonis) larvae from an algae-eating reference species (Acartia tonsa) with a sensitivity of up to 99%. The system successfully differentiates the two species using mixed-species cultures at different ratios. This work shows the potential of fluorescent pest monitoring in the salmon farming industry and paves the way for single-ended aquatic lidars.</p>}},
author = {{Nielsen, J. H. and Pedersen, C. and Kiørboe, T. and Nikolajsen, T. and Brydegaard, M. and Rodrigo, P. J.}},
issn = {{2578-7519}},
language = {{eng}},
number = {{7}},
pages = {{1730--1738}},
publisher = {{Optical Society of America}},
series = {{OSA Continuum}},
title = {{Dual-band fluorosensor for discriminating non-eating from algae-eating zooplankton in aquatic environments}},
url = {{http://dx.doi.org/10.1364/OSAC.389089}},
doi = {{10.1364/OSAC.389089}},
volume = {{3}},
year = {{2020}},
}