Prospective study of relationship between cytomegalovirus pneumonia and viral load in renal transplant recipients
(2004) In Transplantation Proceedings 36(10). p.3036-3041- Abstract
- The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes... (More)
- The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes among both groups. From week 5 (week 4, in leukocytes), plasma CMV-DNA loads in the CMV-IP group increased, the peak value reached at week 8 in plasma and the week 9 in leukocytes. Whereas, the CMV-DNA loads both in plasma and in leukocytes in the non-CMV-IP group fluctuated at lower levels, those in plasma were significantly different between the 2 groups at the weeks 5, 7, and 9. For CMV-DNA in leukocytes, there were significant differences between 2 groups from week 6 to week 11. The present study demonstrated that dynamic determination of CMV-DNA may predict the occurrence of CMV-IP. Viral loads over 10(4) copies/mL plasma continuing for 3 weeks may serve as a cutoff to predict CMV-IP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/254028
- author
- Ye, Q ; Luo, G ; He, X ; Zheng, W ; Zheng, L ; Dong, X ; Xu, X ; Nilsson-Ehle, Peter LU and Xu, Ning LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transplantation Proceedings
- volume
- 36
- issue
- 10
- pages
- 3036 - 3041
- publisher
- Elsevier
- external identifiers
-
- pmid:15686689
- wos:000226765800039
- scopus:21244458875
- pmid:15686689
- ISSN
- 0041-1345
- DOI
- 10.1016/j.transproceed.2004.10.050
- language
- English
- LU publication?
- yes
- id
- d1c5506f-d9da-4bbd-b9b6-c985b746f31f (old id 254028)
- date added to LUP
- 2016-04-01 11:35:24
- date last changed
- 2022-01-26 07:20:42
@article{d1c5506f-d9da-4bbd-b9b6-c985b746f31f, abstract = {{The present study prospectively examined the relationship between cytomegalovirus interstitial pneumonia (CMV-IP) and viral load among 56 renal transplant recipients. We sought to identify the cutoff of viral load to predict CMV-IP. Blood samples were obtained weekly within the first 2 months and every second week during 2 to 6 months after kidney transplantations. A commercial real-time polymerase chain reaction (PCR)-method was applied to quantify CMV-DNA in plasma or in leukocytes. Among 54 renal transplant recipients who were analyzed for CMV-DNA in the blood (96.4%), 8 experienced CMV-IP (14.3%) and 2 died (3.6%). After kidney transplantation, CMV-DNA loads were near 0 in plasma before the week 4 and before the week 3 in leukocytes among both groups. From week 5 (week 4, in leukocytes), plasma CMV-DNA loads in the CMV-IP group increased, the peak value reached at week 8 in plasma and the week 9 in leukocytes. Whereas, the CMV-DNA loads both in plasma and in leukocytes in the non-CMV-IP group fluctuated at lower levels, those in plasma were significantly different between the 2 groups at the weeks 5, 7, and 9. For CMV-DNA in leukocytes, there were significant differences between 2 groups from week 6 to week 11. The present study demonstrated that dynamic determination of CMV-DNA may predict the occurrence of CMV-IP. Viral loads over 10(4) copies/mL plasma continuing for 3 weeks may serve as a cutoff to predict CMV-IP.}}, author = {{Ye, Q and Luo, G and He, X and Zheng, W and Zheng, L and Dong, X and Xu, X and Nilsson-Ehle, Peter and Xu, Ning}}, issn = {{0041-1345}}, language = {{eng}}, number = {{10}}, pages = {{3036--3041}}, publisher = {{Elsevier}}, series = {{Transplantation Proceedings}}, title = {{Prospective study of relationship between cytomegalovirus pneumonia and viral load in renal transplant recipients}}, url = {{http://dx.doi.org/10.1016/j.transproceed.2004.10.050}}, doi = {{10.1016/j.transproceed.2004.10.050}}, volume = {{36}}, year = {{2004}}, }