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Regulation of cell-cell adhesion in prostate cancer cells by microRNA-96 through upregulation of E-Cadherin and EpCAM

Voss, Gjendine LU ; Haflidadóttir, Benedikta S. LU ; Järemo, Helena ; Persson, Margareta LU ; Catela Ivkovic, Tina LU ; Wikström, Pernilla and Ceder, Yvonne LU orcid (2020) In Carcinogenesis 41(7). p.865-874
Abstract

Prostate cancer is one of the most common cancers in men, yet the biology behind lethal disease progression and bone metastasis is poorly understood. In this study, we found elevated levels of microRNA-96 (miR-96) in prostate cancer bone metastasis samples. To determine the molecular mechanisms by which miR-96 deregulation contributes to metastatic progression, we performed an Argonaute2-immunoprecipitation assay, in which mRNAs associated with cell-cell interaction were enriched. The expression of two cell adhesion molecules, E-Cadherin and EpCAM, was upregulated by miR-96, and potential targets sites were identified in the coding sequences of their mRNAs. We further showed that miR-96 enhanced cell-cell adhesion between prostate... (More)

Prostate cancer is one of the most common cancers in men, yet the biology behind lethal disease progression and bone metastasis is poorly understood. In this study, we found elevated levels of microRNA-96 (miR-96) in prostate cancer bone metastasis samples. To determine the molecular mechanisms by which miR-96 deregulation contributes to metastatic progression, we performed an Argonaute2-immunoprecipitation assay, in which mRNAs associated with cell-cell interaction were enriched. The expression of two cell adhesion molecules, E-Cadherin and EpCAM, was upregulated by miR-96, and potential targets sites were identified in the coding sequences of their mRNAs. We further showed that miR-96 enhanced cell-cell adhesion between prostate cancer cells as well as their ability to bind to osteoblasts. Our findings suggest that increased levels of miR-96 give prostate cancer cells an advantage at forming metastases in the bone microenvironment due to increased cell-cell interaction. We propose that miR-96 promotes bone metastasis in prostate cancer patients by facilitating the outgrowth of macroscopic tumours in the bone.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Carcinogenesis
volume
41
issue
7
pages
10 pages
publisher
Oxford University Press
external identifiers
  • pmid:31738404
  • scopus:85088176847
ISSN
0143-3334
DOI
10.1093/carcin/bgz191
language
English
LU publication?
yes
id
d1d9697b-0341-4ace-9e5f-1a0bd3a74020
date added to LUP
2020-07-29 09:54:05
date last changed
2024-04-17 12:54:04
@article{d1d9697b-0341-4ace-9e5f-1a0bd3a74020,
  abstract     = {{<p>Prostate cancer is one of the most common cancers in men, yet the biology behind lethal disease progression and bone metastasis is poorly understood. In this study, we found elevated levels of microRNA-96 (miR-96) in prostate cancer bone metastasis samples. To determine the molecular mechanisms by which miR-96 deregulation contributes to metastatic progression, we performed an Argonaute2-immunoprecipitation assay, in which mRNAs associated with cell-cell interaction were enriched. The expression of two cell adhesion molecules, E-Cadherin and EpCAM, was upregulated by miR-96, and potential targets sites were identified in the coding sequences of their mRNAs. We further showed that miR-96 enhanced cell-cell adhesion between prostate cancer cells as well as their ability to bind to osteoblasts. Our findings suggest that increased levels of miR-96 give prostate cancer cells an advantage at forming metastases in the bone microenvironment due to increased cell-cell interaction. We propose that miR-96 promotes bone metastasis in prostate cancer patients by facilitating the outgrowth of macroscopic tumours in the bone.</p>}},
  author       = {{Voss, Gjendine and Haflidadóttir, Benedikta S. and Järemo, Helena and Persson, Margareta and Catela Ivkovic, Tina and Wikström, Pernilla and Ceder, Yvonne}},
  issn         = {{0143-3334}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{865--874}},
  publisher    = {{Oxford University Press}},
  series       = {{Carcinogenesis}},
  title        = {{Regulation of cell-cell adhesion in prostate cancer cells by microRNA-96 through upregulation of E-Cadherin and EpCAM}},
  url          = {{http://dx.doi.org/10.1093/carcin/bgz191}},
  doi          = {{10.1093/carcin/bgz191}},
  volume       = {{41}},
  year         = {{2020}},
}