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Production of bio-based adipic acid using a combination of engineered Pseudomonas putida strains

Lund, Fredrik LU and Gorwa-Grauslund, Marie LU (2024) In Sustainable Chemistry for the Environment 8.
Abstract
Adipic acid is an industrially important dicarboxylic acid and an
essential precursor in the synthesis of Nylon-6,6. Due to the
environmental impact associated with the current synthetic methods for
Nylon-6,6 production, greener alternatives based on renewable and
bio-based feedstocks are needed. In the present study, the potential of
two Pseudomonas putida strains engineered for muconic acid
accumulation was used to produce adipic acid from the lignin-derived
aromatic compound guaiacol. This was achieved by expressing genes
enabling the conversion of guaiacol to catechol in the first strain, and
the conversion of muconic acid to adipic acid via expression of the Bacillus... (More)
Adipic acid is an industrially important dicarboxylic acid and an
essential precursor in the synthesis of Nylon-6,6. Due to the
environmental impact associated with the current synthetic methods for
Nylon-6,6 production, greener alternatives based on renewable and
bio-based feedstocks are needed. In the present study, the potential of
two Pseudomonas putida strains engineered for muconic acid
accumulation was used to produce adipic acid from the lignin-derived
aromatic compound guaiacol. This was achieved by expressing genes
enabling the conversion of guaiacol to catechol in the first strain, and
the conversion of muconic acid to adipic acid via expression of the Bacillus coagulans
enoate reductase gene in the second strain. Through process
engineering, a 2-step production was designed in which 2.93 mM
bio-adipic acid was produced, with a yield of 0.57 mol/mol. This is the
first demonstration that process and strain engineering can be combined
to obtain a complete bio-based route for adipic acid production from
depolymerized lignin compounds using the robust P. putida chassis. (Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Sustainable Chemistry for the Environment
volume
8
article number
100154
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85203139981
ISSN
2949-8392
DOI
10.1016/j.scenv.2024.100154
language
English
LU publication?
yes
id
d215b9ba-b1fd-4515-bf07-8c9fe93451aa
date added to LUP
2024-10-16 11:54:04
date last changed
2024-10-17 14:36:32
@article{d215b9ba-b1fd-4515-bf07-8c9fe93451aa,
  abstract     = {{Adipic acid is an industrially important dicarboxylic acid and an <br>
essential precursor in the synthesis of Nylon-6,6. Due to the <br>
environmental impact associated with the current synthetic methods for <br>
Nylon-6,6 production, greener alternatives based on renewable and <br>
bio-based feedstocks are needed. In the present study, the potential of <br>
two <em>Pseudomonas putida</em> strains engineered for muconic acid <br>
accumulation was used to produce adipic acid from the lignin-derived <br>
aromatic compound guaiacol. This was achieved by expressing genes <br>
enabling the conversion of guaiacol to catechol in the first strain, and<br>
 the conversion of muconic acid to adipic acid via expression of the <em>Bacillus coagulans</em><br>
 enoate reductase gene in the second strain. Through process <br>
engineering, a 2-step production was designed in which 2.93 mM <br>
bio-adipic acid was produced, with a yield of 0.57 mol/mol. This is the <br>
first demonstration that process and strain engineering can be combined <br>
to obtain a complete bio-based route for adipic acid production from <br>
depolymerized lignin compounds using the robust <em>P. putida</em> chassis.}},
  author       = {{Lund, Fredrik and Gorwa-Grauslund, Marie}},
  issn         = {{2949-8392}},
  language     = {{eng}},
  month        = {{09}},
  publisher    = {{Elsevier}},
  series       = {{Sustainable Chemistry for the Environment}},
  title        = {{Production of bio-based adipic acid using a combination of engineered <i>Pseudomonas putida </i>strains}},
  url          = {{http://dx.doi.org/10.1016/j.scenv.2024.100154}},
  doi          = {{10.1016/j.scenv.2024.100154}},
  volume       = {{8}},
  year         = {{2024}},
}