Advanced

Histidine Containing Macroporous Affinity Cryogels for Immunoglobulin G Purification

Bereli, Nilay ; Ertürk, Gizem LU and Denizli, Adil (2012) In Separation Science and Technology (Philadelphia) 47(12). p.1813-1820
Abstract

A supermacroporous cryogel was prepared to obtain an efficient and cost effective purification of IgG from human plasma. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the pseudospecific ligand and/or comonomer. Poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methylester) [PHEMAH] cryogel was produced by free radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. PHEMAH cryogel had a specific surface area of 38.6 m 2/g. PHEMAH cryogel was characterized by swelling studies, scanning electron microscopy and elemental analysis. PHEMAH cryogel containing 113.7 μmol MAH/g was used in the purification of IgG from human plasma.... (More)

A supermacroporous cryogel was prepared to obtain an efficient and cost effective purification of IgG from human plasma. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the pseudospecific ligand and/or comonomer. Poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methylester) [PHEMAH] cryogel was produced by free radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. PHEMAH cryogel had a specific surface area of 38.6 m 2/g. PHEMAH cryogel was characterized by swelling studies, scanning electron microscopy and elemental analysis. PHEMAH cryogel containing 113.7 μmol MAH/g was used in the purification of IgG from human plasma. Compared with the poly(hydroxyethyl methacrylate) (PHEMA) cryogel (0.4 mg/g), the IgG adsorption capacity of the PHEMAH cryogel (24.7 mg/g) was improved significantly due to the MAH incorporation into the polymeric matrix. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was observed at pH 7.4. The IgG adsorption amount from human plasma was 97.3 mg/g with a purity of 94.6%. It was observed that IgG could be repeatedly adsorbed and eluted with PHEMAH cryogel without significant loss in the adsorption capacity.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
antibody separation, cryogels, IgG, protein purification, pseudospecific adsorbents
in
Separation Science and Technology (Philadelphia)
volume
47
issue
12
pages
8 pages
publisher
Marcel Dekker
external identifiers
  • scopus:84864687678
ISSN
0149-6395
DOI
10.1080/01496395.2012.662258
language
English
LU publication?
no
id
d2452ce4-3ecd-4971-b216-02f44470a1bb
date added to LUP
2018-01-25 14:00:58
date last changed
2020-05-13 04:45:57
@article{d2452ce4-3ecd-4971-b216-02f44470a1bb,
  abstract     = {<p>A supermacroporous cryogel was prepared to obtain an efficient and cost effective purification of IgG from human plasma. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the pseudospecific ligand and/or comonomer. Poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methylester) [PHEMAH] cryogel was produced by free radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. PHEMAH cryogel had a specific surface area of 38.6 m <sup>2</sup>/g. PHEMAH cryogel was characterized by swelling studies, scanning electron microscopy and elemental analysis. PHEMAH cryogel containing 113.7 μmol MAH/g was used in the purification of IgG from human plasma. Compared with the poly(hydroxyethyl methacrylate) (PHEMA) cryogel (0.4 mg/g), the IgG adsorption capacity of the PHEMAH cryogel (24.7 mg/g) was improved significantly due to the MAH incorporation into the polymeric matrix. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was observed at pH 7.4. The IgG adsorption amount from human plasma was 97.3 mg/g with a purity of 94.6%. It was observed that IgG could be repeatedly adsorbed and eluted with PHEMAH cryogel without significant loss in the adsorption capacity.</p>},
  author       = {Bereli, Nilay and Ertürk, Gizem and Denizli, Adil},
  issn         = {0149-6395},
  language     = {eng},
  number       = {12},
  pages        = {1813--1820},
  publisher    = {Marcel Dekker},
  series       = {Separation Science and Technology (Philadelphia)},
  title        = {Histidine Containing Macroporous Affinity Cryogels for Immunoglobulin G Purification},
  url          = {http://dx.doi.org/10.1080/01496395.2012.662258},
  doi          = {10.1080/01496395.2012.662258},
  volume       = {47},
  year         = {2012},
}