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A theoretical treatment of damped oscillations in the transient state kinetics of single‐enzyme reactions

Ryde-Pettersson, Ulf LU (1989) In European Journal of Biochemistry 186(1-2). p.145-148
Abstract

An extension of the available kinetic theory for reactions in the transient state is presented which establishes that single‐enzyme reactions may exhibit damped oscillations under the conditions of standard kinetic experiments performed by stopped‐flow techniques. Such oscillations may occur for reasonable magnitudes of rate constants in the enzymic reaction mechanism and at physiological concentrations of enzyme and substrate. In the simplest reaction systems, the oscillations will be strongly damped and lead to progress curves resembling those of a reaction governed by standard exponential transients; statistical regression methods may then have to be applied for their detection and characterization. The observation that single‐enzyme... (More)

An extension of the available kinetic theory for reactions in the transient state is presented which establishes that single‐enzyme reactions may exhibit damped oscillations under the conditions of standard kinetic experiments performed by stopped‐flow techniques. Such oscillations may occur for reasonable magnitudes of rate constants in the enzymic reaction mechanism and at physiological concentrations of enzyme and substrate. In the simplest reaction systems, the oscillations will be strongly damped and lead to progress curves resembling those of a reaction governed by standard exponential transients; statistical regression methods may then have to be applied for their detection and characterization. The observation that single‐enzyme reactions may exhibit oscillatory behaviour points to a previously unrecognized possible source of the damped oscillations observed in metabolic systems such as the pathways of glycolysis or photosynthesis. Copyright © 1989, Wiley Blackwell. All rights reserved

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Biochemistry
volume
186
issue
1-2
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024818508
ISSN
0014-2956
DOI
10.1111/j.1432-1033.1989.tb15188.x
language
English
LU publication?
yes
id
d26292be-1928-47d0-938f-334cf556d5d7
date added to LUP
2017-02-04 11:21:07
date last changed
2017-05-01 20:30:08
@article{d26292be-1928-47d0-938f-334cf556d5d7,
  abstract     = {<p>An extension of the available kinetic theory for reactions in the transient state is presented which establishes that single‐enzyme reactions may exhibit damped oscillations under the conditions of standard kinetic experiments performed by stopped‐flow techniques. Such oscillations may occur for reasonable magnitudes of rate constants in the enzymic reaction mechanism and at physiological concentrations of enzyme and substrate. In the simplest reaction systems, the oscillations will be strongly damped and lead to progress curves resembling those of a reaction governed by standard exponential transients; statistical regression methods may then have to be applied for their detection and characterization. The observation that single‐enzyme reactions may exhibit oscillatory behaviour points to a previously unrecognized possible source of the damped oscillations observed in metabolic systems such as the pathways of glycolysis or photosynthesis. Copyright © 1989, Wiley Blackwell. All rights reserved</p>},
  author       = {Ryde-Pettersson, Ulf},
  issn         = {0014-2956},
  language     = {eng},
  number       = {1-2},
  pages        = {145--148},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {A theoretical treatment of damped oscillations in the transient state kinetics of single‐enzyme reactions},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.1989.tb15188.x},
  volume       = {186},
  year         = {1989},
}