Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.
Feás, Xesús ; Ye, Lei LU
; Hosseini, Seyed V
; Fente, Cristina A
and Cepeda, Alberto
(2009)
In Journal of Pharmaceutical and Biomedical Analysis
50.
p.1044-1049
- Abstract
- A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was... (More)
- A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1453442
- author
- Feás, Xesús
; Ye, Lei
LU
; Hosseini, Seyed V
; Fente, Cristina A
and Cepeda, Alberto
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Pharmaceutical and Biomedical Analysis
- volume
- 50
- pages
- 1044 - 1049
- publisher
- Elsevier
- external identifiers
-
- wos:000270759600054
- pmid:19574011
- scopus:70349189156
- ISSN
- 0731-7085
- DOI
- 10.1016/j.jpba.2009.06.006
- language
- English
- LU publication?
- yes
- id
- d27f7ab4-05d9-4727-a3de-86f8164a5ba1 (old id 1453442)
- date added to LUP
- 2016-04-01 12:14:43
- date last changed
- 2022-01-27 00:55:16
@article{d27f7ab4-05d9-4727-a3de-86f8164a5ba1,
abstract = {{A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations.}},
author = {{Feás, Xesús and Ye, Lei and Hosseini, Seyed V and Fente, Cristina A and Cepeda, Alberto}},
issn = {{0731-7085}},
language = {{eng}},
pages = {{1044--1049}},
publisher = {{Elsevier}},
series = {{Journal of Pharmaceutical and Biomedical Analysis}},
title = {{Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.}},
url = {{http://dx.doi.org/10.1016/j.jpba.2009.06.006}},
doi = {{10.1016/j.jpba.2009.06.006}},
volume = {{50}},
year = {{2009}},
}