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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

Bergdahl, Gizem Ertürk LU ; Hedström, Martin LU and Mattiasson, Bo LU (2019) In Applied Biochemistry and Biotechnology 189. p.374-383
Abstract

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capacitive sensor, Degradation, Enzymatic activity, Protease
in
Applied Biochemistry and Biotechnology
volume
189
pages
10 pages
publisher
Humana Press
external identifiers
  • pmid:31020512
  • scopus:85064908179
ISSN
0273-2289
DOI
10.1007/s12010-019-03006-0
language
English
LU publication?
yes
id
d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d
date added to LUP
2019-05-17 11:53:39
date last changed
2024-06-25 13:53:11
@article{d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d,
  abstract     = {{<p>A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.</p>}},
  author       = {{Bergdahl, Gizem Ertürk and Hedström, Martin and Mattiasson, Bo}},
  issn         = {{0273-2289}},
  keywords     = {{Capacitive sensor; Degradation; Enzymatic activity; Protease}},
  language     = {{eng}},
  pages        = {{374--383}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time}},
  url          = {{http://dx.doi.org/10.1007/s12010-019-03006-0}},
  doi          = {{10.1007/s12010-019-03006-0}},
  volume       = {{189}},
  year         = {{2019}},
}