Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
(2019) In Applied Biochemistry and Biotechnology 189. p.374-383- Abstract
A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.
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https://lup.lub.lu.se/record/d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d
- author
- Bergdahl, Gizem Ertürk LU ; Hedström, Martin LU and Mattiasson, Bo LU
- organization
- publishing date
- 2019-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Capacitive sensor, Degradation, Enzymatic activity, Protease
- in
- Applied Biochemistry and Biotechnology
- volume
- 189
- pages
- 10 pages
- publisher
- Humana Press
- external identifiers
-
- scopus:85064908179
- pmid:31020512
- ISSN
- 0273-2289
- DOI
- 10.1007/s12010-019-03006-0
- language
- English
- LU publication?
- yes
- id
- d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d
- date added to LUP
- 2019-05-17 11:53:39
- date last changed
- 2024-08-07 17:24:16
@article{d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d, abstract = {{<p>A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.</p>}}, author = {{Bergdahl, Gizem Ertürk and Hedström, Martin and Mattiasson, Bo}}, issn = {{0273-2289}}, keywords = {{Capacitive sensor; Degradation; Enzymatic activity; Protease}}, language = {{eng}}, pages = {{374--383}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology}}, title = {{Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time}}, url = {{http://dx.doi.org/10.1007/s12010-019-03006-0}}, doi = {{10.1007/s12010-019-03006-0}}, volume = {{189}}, year = {{2019}}, }