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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

Bergdahl, Gizem Ertürk LU ; Hedström, Martin LU and Mattiasson, Bo LU (2019) In Applied Biochemistry and Biotechnology
Abstract

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Capacitive sensor, Degradation, Enzymatic activity, Protease
in
Applied Biochemistry and Biotechnology
publisher
Humana Press
external identifiers
  • scopus:85064908179
ISSN
0273-2289
DOI
10.1007/s12010-019-03006-0
language
English
LU publication?
yes
id
d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d
date added to LUP
2019-05-17 11:53:39
date last changed
2019-06-04 03:58:47
@article{d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d,
  abstract     = {<p>A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.</p>},
  author       = {Bergdahl, Gizem Ertürk and Hedström, Martin and Mattiasson, Bo},
  issn         = {0273-2289},
  keyword      = {Capacitive sensor,Degradation,Enzymatic activity,Protease},
  language     = {eng},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time},
  url          = {http://dx.doi.org/10.1007/s12010-019-03006-0},
  year         = {2019},
}