Measuring and Analyzing Binding Kinetics of Coupled Folding and Binding Reactions Under Pseudo-First-Order Conditions
(2020) In Methods in molecular biology (Clifton, N.J.) 2141. p.629-650- Abstract
Many intrinsically disordered proteins (IDPs) adopt a well-defined structure upon binding to their interaction partners. Kinetic characterization is a requirement for the investigation of the dynamics and mechanisms of these folding-upon-binding reactions. Here a protocol is described for the investigation of binding kinetics of bimolecular binding and folding reactions of IDPs to their ligand partner under pseudo-first-order conditions using stopped-flow mixing and fluorescence detection.
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- author
- Jensen, Kristine Steen LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Association and dissociation rate constants, Binding mechanisms, Displacement kinetics, Folding and binding, IDP, Intrinsically disordered proteins, Stopped-flow measurements
- in
- Methods in molecular biology (Clifton, N.J.)
- volume
- 2141
- pages
- 22 pages
- publisher
- Springer
- external identifiers
-
- pmid:32696381
- scopus:85088523295
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-0716-0524-0_32
- language
- English
- LU publication?
- yes
- id
- d2e265c0-3d27-485d-85aa-1069896a0938
- date added to LUP
- 2020-08-05 12:55:20
- date last changed
- 2024-09-05 04:23:35
@article{d2e265c0-3d27-485d-85aa-1069896a0938, abstract = {{<p>Many intrinsically disordered proteins (IDPs) adopt a well-defined structure upon binding to their interaction partners. Kinetic characterization is a requirement for the investigation of the dynamics and mechanisms of these folding-upon-binding reactions. Here a protocol is described for the investigation of binding kinetics of bimolecular binding and folding reactions of IDPs to their ligand partner under pseudo-first-order conditions using stopped-flow mixing and fluorescence detection.</p>}}, author = {{Jensen, Kristine Steen}}, issn = {{1940-6029}}, keywords = {{Association and dissociation rate constants; Binding mechanisms; Displacement kinetics; Folding and binding; IDP; Intrinsically disordered proteins; Stopped-flow measurements}}, language = {{eng}}, pages = {{629--650}}, publisher = {{Springer}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Measuring and Analyzing Binding Kinetics of Coupled Folding and Binding Reactions Under Pseudo-First-Order Conditions}}, url = {{http://dx.doi.org/10.1007/978-1-0716-0524-0_32}}, doi = {{10.1007/978-1-0716-0524-0_32}}, volume = {{2141}}, year = {{2020}}, }