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Measuring and Analyzing Binding Kinetics of Coupled Folding and Binding Reactions Under Pseudo-First-Order Conditions

Jensen, Kristine Steen LU (2020) In Methods in molecular biology (Clifton, N.J.) 2141. p.629-650
Abstract

Many intrinsically disordered proteins (IDPs) adopt a well-defined structure upon binding to their interaction partners. Kinetic characterization is a requirement for the investigation of the dynamics and mechanisms of these folding-upon-binding reactions. Here a protocol is described for the investigation of binding kinetics of bimolecular binding and folding reactions of IDPs to their ligand partner under pseudo-first-order conditions using stopped-flow mixing and fluorescence detection.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Association and dissociation rate constants, Binding mechanisms, Displacement kinetics, Folding and binding, IDP, Intrinsically disordered proteins, Stopped-flow measurements
in
Methods in molecular biology (Clifton, N.J.)
volume
2141
pages
22 pages
publisher
Springer
external identifiers
  • scopus:85088523295
  • pmid:32696381
ISSN
1940-6029
DOI
10.1007/978-1-0716-0524-0_32
language
English
LU publication?
yes
id
d2e265c0-3d27-485d-85aa-1069896a0938
date added to LUP
2020-08-05 12:55:20
date last changed
2024-04-03 13:09:37
@article{d2e265c0-3d27-485d-85aa-1069896a0938,
  abstract     = {{<p>Many intrinsically disordered proteins (IDPs) adopt a well-defined structure upon binding to their interaction partners. Kinetic characterization is a requirement for the investigation of the dynamics and mechanisms of these folding-upon-binding reactions. Here a protocol is described for the investigation of binding kinetics of bimolecular binding and folding reactions of IDPs to their ligand partner under pseudo-first-order conditions using stopped-flow mixing and fluorescence detection.</p>}},
  author       = {{Jensen, Kristine Steen}},
  issn         = {{1940-6029}},
  keywords     = {{Association and dissociation rate constants; Binding mechanisms; Displacement kinetics; Folding and binding; IDP; Intrinsically disordered proteins; Stopped-flow measurements}},
  language     = {{eng}},
  pages        = {{629--650}},
  publisher    = {{Springer}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Measuring and Analyzing Binding Kinetics of Coupled Folding and Binding Reactions Under Pseudo-First-Order Conditions}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-0524-0_32}},
  doi          = {{10.1007/978-1-0716-0524-0_32}},
  volume       = {{2141}},
  year         = {{2020}},
}