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Analysis of the interaction of Aeromonas caviae, A. hydrophila and A. sobria with mucins

Ascencio, F ; Martinez Arias, Wilma LU ; Romero, MJ and Wadström, Torkel LU (1998) In FEMS Immunol Med Microbiol. 20(3). p.29-219
Abstract
Aeromonas species are known to be involved in human gastrointestinal diseases. These organisms colonize the gastrointestinal tract. Aeromonas hydrophila, A. caviae, and A. sobria have been demonstrated microscopically to adhere to animal cell lines that express mucous receptors, but quantitative studies of adherence to mucosal components such as mucin have not been published to date. Purified bovine submaxillary gland, hog gastric mucin, and fish skin mucin were used as a model to study mucin-binding activity among A. caviae, A. hydrophila, and A. sobria strains. Our findings revealed that binding of radiolabeled and enzyme-conjugated mucins to Aeromonas cells varied depending on the labeling procedure. The highest binding was observed... (More)
Aeromonas species are known to be involved in human gastrointestinal diseases. These organisms colonize the gastrointestinal tract. Aeromonas hydrophila, A. caviae, and A. sobria have been demonstrated microscopically to adhere to animal cell lines that express mucous receptors, but quantitative studies of adherence to mucosal components such as mucin have not been published to date. Purified bovine submaxillary gland, hog gastric mucin, and fish skin mucin were used as a model to study mucin-binding activity among A. caviae, A. hydrophila, and A. sobria strains. Our findings revealed that binding of radiolabeled and enzyme-conjugated mucins to Aeromonas cells varied depending on the labeling procedure. The highest binding was observed when the three mucin preparations were labeled with horseradish peroxidase. Binding of the various horseradish peroxidase-labeled mucins by A. caviae, A. hydrophila, and A. sobria cells is a common property among Aeromonas species isolated from human infections, diseased fish, and from environmental sources. The proportion of Aeromonas strains which bind the various horseradish peroxidase-labeled mucins was significantly higher for A. hydrophila than for A. caviae and A. sobria. Bacterial cell-surface extracts containing active mucin-binding components recognized the horseradish peroxidase-labeled mucins. The molecular masses of the mucin-binding proteins were estimated by SDS-PAGE and Western blot as follows: A. caviae strain A4812 (95 and 44 kDa); A. hydrophila strain 48748 (97, 45, 33 and 22 kDa); and A. sobria strain 48739 (95 and 43 kDa). Mucin interaction with Aeromonas cells was also studied in terms of growth in mucin-rich media. The culture conditions greatly influence the expression of A. hydrophila mucin-binding activity. (Less)
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keywords
Western Cattle Culture Media Electrophoresis, Polyacrylamide Gel Fishes Gastric Mucins/metabolism Horseradish Peroxidase Humans Immunoblotting Mucins/metabolism* Skin Submandibular Gland/metabolism Swine, Aeromonas/growth & development Aeromonas/metabolism* Aeromonas hydrophila/growth & development Aeromonas hydrophila/metabolism* Animals Bacterial Adhesion Bacterial Proteins/isolation & purification Bacterial Proteins/metabolism* Blotting
in
FEMS Immunol Med Microbiol.
volume
20
issue
3
pages
29 - 219
external identifiers
  • scopus:0031897475
DOI
10.1111/j.1574-695X.1998.tb01130.x
language
English
LU publication?
yes
id
d31041e7-9a34-4af3-a81e-ec1d496d13a5 (old id 1753648)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/9566493
date added to LUP
2016-04-04 14:36:47
date last changed
2022-01-30 02:17:14
@article{d31041e7-9a34-4af3-a81e-ec1d496d13a5,
  abstract     = {{Aeromonas species are known to be involved in human gastrointestinal diseases. These organisms colonize the gastrointestinal tract. Aeromonas hydrophila, A. caviae, and A. sobria have been demonstrated microscopically to adhere to animal cell lines that express mucous receptors, but quantitative studies of adherence to mucosal components such as mucin have not been published to date. Purified bovine submaxillary gland, hog gastric mucin, and fish skin mucin were used as a model to study mucin-binding activity among A. caviae, A. hydrophila, and A. sobria strains. Our findings revealed that binding of radiolabeled and enzyme-conjugated mucins to Aeromonas cells varied depending on the labeling procedure. The highest binding was observed when the three mucin preparations were labeled with horseradish peroxidase. Binding of the various horseradish peroxidase-labeled mucins by A. caviae, A. hydrophila, and A. sobria cells is a common property among Aeromonas species isolated from human infections, diseased fish, and from environmental sources. The proportion of Aeromonas strains which bind the various horseradish peroxidase-labeled mucins was significantly higher for A. hydrophila than for A. caviae and A. sobria. Bacterial cell-surface extracts containing active mucin-binding components recognized the horseradish peroxidase-labeled mucins. The molecular masses of the mucin-binding proteins were estimated by SDS-PAGE and Western blot as follows: A. caviae strain A4812 (95 and 44 kDa); A. hydrophila strain 48748 (97, 45, 33 and 22 kDa); and A. sobria strain 48739 (95 and 43 kDa). Mucin interaction with Aeromonas cells was also studied in terms of growth in mucin-rich media. The culture conditions greatly influence the expression of A. hydrophila mucin-binding activity.}},
  author       = {{Ascencio, F and Martinez Arias, Wilma and Romero, MJ and Wadström, Torkel}},
  keywords     = {{Western
Cattle
Culture Media
Electrophoresis; Polyacrylamide Gel
Fishes
Gastric Mucins/metabolism
Horseradish Peroxidase
Humans
Immunoblotting
Mucins/metabolism*
Skin
Submandibular Gland/metabolism
Swine; Aeromonas/growth & development
Aeromonas/metabolism*
Aeromonas hydrophila/growth & development
Aeromonas hydrophila/metabolism*
Animals
Bacterial Adhesion
Bacterial Proteins/isolation & purification
Bacterial Proteins/metabolism*
Blotting}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{29--219}},
  series       = {{FEMS Immunol Med Microbiol.}},
  title        = {{Analysis of the interaction of Aeromonas caviae, A. hydrophila and A. sobria with mucins}},
  url          = {{http://dx.doi.org/10.1111/j.1574-695X.1998.tb01130.x}},
  doi          = {{10.1111/j.1574-695X.1998.tb01130.x}},
  volume       = {{20}},
  year         = {{1998}},
}