Hematopoietic cells emerging from hemogenic endothelium exhibit lineage-specific oxidative stress responses
Biezeman, Harmke ; Nubiè, Martina and Oburoglu, Leal LU
(2024)
In Journal of Biological Chemistry
300(11).
- Abstract
During human embryogenesis, distinct waves of hematopoiesis give rise to various blood cell types, originating from hemogenic endothelial (HE) cells. As HE cells reside in hypoxic conditions in the embryo, we investigated the role of hypoxia in human endothelial to hematopoietic transition and subsequent hematopoiesis. Using single-cell RNA sequencing, we describe hypoxia-related transcriptional changes in different HE-derived blood lineages, which reveal that erythroid cells are particularly susceptible to oxidative stress, due to decreased NRF2 activity in hypoxia. In contrast, nonerythroid CD45+ cells exhibit increased proliferative rates in hypoxic conditions and enhanced resilience to oxidative stress. We find that even... (More)
During human embryogenesis, distinct waves of hematopoiesis give rise to various blood cell types, originating from hemogenic endothelial (HE) cells. As HE cells reside in hypoxic conditions in the embryo, we investigated the role of hypoxia in human endothelial to hematopoietic transition and subsequent hematopoiesis. Using single-cell RNA sequencing, we describe hypoxia-related transcriptional changes in different HE-derived blood lineages, which reveal that erythroid cells are particularly susceptible to oxidative stress, due to decreased NRF2 activity in hypoxia. In contrast, nonerythroid CD45+ cells exhibit increased proliferative rates in hypoxic conditions and enhanced resilience to oxidative stress. We find that even in normoxia, erythroid cells present a clear predisposition to oxidative stress, with low glutathione levels and high lipid peroxidation, in contrast to CD45+ cells. Intriguingly, reactive oxygen species are produced at different sites in GPA+ and CD45+ cells, revealing differences in oxidative phosphorylation and the use of canonical versus noncanonical tricarboxylic acid cycle in these lineages. Our findings elucidate how hypoxia and oxidative stress distinctly affect HE-derived hematopoietic lineages, uncovering critical transcriptional and metabolic pathways that influence blood cell development.
(Less)
- author
- Biezeman, Harmke
; Nubiè, Martina
and Oburoglu, Leal
LU
- organization
- publishing date
- 2024-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- endothelial to hematopoietic transition, erythropoiesis, glutathione, hematopoiesis, hypoxia, induced pluripotent stem cells, Nrf2, oxidative stress
- in
- Journal of Biological Chemistry
- volume
- 300
- issue
- 11
- article number
- 107815
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:85206821345
- pmid:39326495
- ISSN
- 0021-9258
- DOI
- 10.1016/j.jbc.2024.107815
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2024 The Authors
- id
- d329483b-f7c9-44fd-8062-998881d294de
- date added to LUP
- 2024-12-18 08:57:34
- date last changed
- 2025-07-31 03:26:52
@article{d329483b-f7c9-44fd-8062-998881d294de,
abstract = {{<p>During human embryogenesis, distinct waves of hematopoiesis give rise to various blood cell types, originating from hemogenic endothelial (HE) cells. As HE cells reside in hypoxic conditions in the embryo, we investigated the role of hypoxia in human endothelial to hematopoietic transition and subsequent hematopoiesis. Using single-cell RNA sequencing, we describe hypoxia-related transcriptional changes in different HE-derived blood lineages, which reveal that erythroid cells are particularly susceptible to oxidative stress, due to decreased NRF2 activity in hypoxia. In contrast, nonerythroid CD45<sup>+</sup> cells exhibit increased proliferative rates in hypoxic conditions and enhanced resilience to oxidative stress. We find that even in normoxia, erythroid cells present a clear predisposition to oxidative stress, with low glutathione levels and high lipid peroxidation, in contrast to CD45<sup>+</sup> cells. Intriguingly, reactive oxygen species are produced at different sites in GPA<sup>+</sup> and CD45<sup>+</sup> cells, revealing differences in oxidative phosphorylation and the use of canonical versus noncanonical tricarboxylic acid cycle in these lineages. Our findings elucidate how hypoxia and oxidative stress distinctly affect HE-derived hematopoietic lineages, uncovering critical transcriptional and metabolic pathways that influence blood cell development.</p>}},
author = {{Biezeman, Harmke and Nubiè, Martina and Oburoglu, Leal}},
issn = {{0021-9258}},
keywords = {{endothelial to hematopoietic transition; erythropoiesis; glutathione; hematopoiesis; hypoxia; induced pluripotent stem cells; Nrf2; oxidative stress}},
language = {{eng}},
number = {{11}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{Hematopoietic cells emerging from hemogenic endothelium exhibit lineage-specific oxidative stress responses}},
url = {{http://dx.doi.org/10.1016/j.jbc.2024.107815}},
doi = {{10.1016/j.jbc.2024.107815}},
volume = {{300}},
year = {{2024}},
}