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Double homozygous waltzer and Ames waltzer mice provide no evidence of retinal degeneration

Ahmed, Zubair M; Kjellstrom, Sten LU ; Haywood-Watson, Ricky J L; Bush, Ronald A; Hampton, Lori L; Battey, James F; Riazuddin, Saima; Frolenkov, Gregory; Sieving, Paul A. and Friedman, Thomas B (2008) In Molecular Vision 14. p.36-2227
Abstract

PURPOSE: To determine whether cadherin 23 and protocadherin 15 can substitute for one another in the maintenance of the retina and other tissues in the mouse. Does homozygosity for both v and av mutant alleles (i.e., a double homozygous mouse) cause retinal degeneration or an obvious retinal histopathology?

METHODS: We generated mice homozygous for both Cdh23(v-6J) and Pcdh15(av-Jfb) alleles. The retinal phenotypes of double heterozygous and double homozygous mutant mice were determined by light microscopy and electroretinography (ERG). Histology on 32 different tissues, scanning electron microscopy of organ of Corti hair cells as well as serum biochemical and hematological examinations were evaluated.

RESULTS: ERG waves of... (More)

PURPOSE: To determine whether cadherin 23 and protocadherin 15 can substitute for one another in the maintenance of the retina and other tissues in the mouse. Does homozygosity for both v and av mutant alleles (i.e., a double homozygous mouse) cause retinal degeneration or an obvious retinal histopathology?

METHODS: We generated mice homozygous for both Cdh23(v-6J) and Pcdh15(av-Jfb) alleles. The retinal phenotypes of double heterozygous and double homozygous mutant mice were determined by light microscopy and electroretinography (ERG). Histology on 32 different tissues, scanning electron microscopy of organ of Corti hair cells as well as serum biochemical and hematological examinations were evaluated.

RESULTS: ERG waves of double heterozygous and double homozygous mice showed similar shape, growth of the amplitude with intensity, and implicit time for both rod and cone pathway mediated responses. Mice homozygous for both Cdh23(v-6J) and Pcdh15(av-Jfb) mutations showed no sign of retinitis pigmentosa or photoreceptor degeneration but, as expected, were deaf and had disorganized hair cell sensory bundles.

CONCLUSIONS: The simultaneous presence of homozygous mutant alleles of cadherin 23 and protocadherin 15 results only in deafness, not retinal degeneration or any other additional obvious phenotype of the major organ systems. We conclude that in the mouse cadherin 23 or protocadherin 15 appear not to compensate for one another to maintain the retina.

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Contribution to journal
publication status
published
keywords
Alleles, Alternative Splicing, Animals, Cadherins, Cell Nucleus, Cilia, Electroretinography, Eye, Heterozygote, Homozygote, Mice, Mice, Mutant Strains, Phenotype, Protein Precursors, Retinal Degeneration, Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural
in
Molecular Vision
volume
14
pages
10 pages
publisher
Molecular Vision
external identifiers
  • scopus:57749169076
ISSN
1090-0535
language
English
LU publication?
no
id
d3565ec8-5e47-422d-9acd-31db065e398a
date added to LUP
2017-04-14 08:32:32
date last changed
2017-04-25 15:24:29
@article{d3565ec8-5e47-422d-9acd-31db065e398a,
  abstract     = {<p>PURPOSE: To determine whether cadherin 23 and protocadherin 15 can substitute for one another in the maintenance of the retina and other tissues in the mouse. Does homozygosity for both v and av mutant alleles (i.e., a double homozygous mouse) cause retinal degeneration or an obvious retinal histopathology?</p><p>METHODS: We generated mice homozygous for both Cdh23(v-6J) and Pcdh15(av-Jfb) alleles. The retinal phenotypes of double heterozygous and double homozygous mutant mice were determined by light microscopy and electroretinography (ERG). Histology on 32 different tissues, scanning electron microscopy of organ of Corti hair cells as well as serum biochemical and hematological examinations were evaluated.</p><p>RESULTS: ERG waves of double heterozygous and double homozygous mice showed similar shape, growth of the amplitude with intensity, and implicit time for both rod and cone pathway mediated responses. Mice homozygous for both Cdh23(v-6J) and Pcdh15(av-Jfb) mutations showed no sign of retinitis pigmentosa or photoreceptor degeneration but, as expected, were deaf and had disorganized hair cell sensory bundles.</p><p>CONCLUSIONS: The simultaneous presence of homozygous mutant alleles of cadherin 23 and protocadherin 15 results only in deafness, not retinal degeneration or any other additional obvious phenotype of the major organ systems. We conclude that in the mouse cadherin 23 or protocadherin 15 appear not to compensate for one another to maintain the retina.</p>},
  author       = {Ahmed, Zubair M and Kjellstrom, Sten and Haywood-Watson, Ricky J L and Bush, Ronald A and Hampton, Lori L and Battey, James F and Riazuddin, Saima and Frolenkov, Gregory and Sieving, Paul A. and Friedman, Thomas B},
  issn         = {1090-0535},
  keyword      = {Alleles,Alternative Splicing,Animals,Cadherins,Cell Nucleus,Cilia,Electroretinography,Eye,Heterozygote,Homozygote,Mice,Mice, Mutant Strains,Phenotype,Protein Precursors,Retinal Degeneration,Journal Article,Research Support, N.I.H., Extramural,Research Support, N.I.H., Intramural},
  language     = {eng},
  pages        = {36--2227},
  publisher    = {Molecular Vision},
  series       = {Molecular Vision},
  title        = {Double homozygous waltzer and Ames waltzer mice provide no evidence of retinal degeneration},
  volume       = {14},
  year         = {2008},
}