High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.
(2015) In PLoS ONE 10(8).- Abstract
- The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the... (More)
- The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7794134
- author
- Nasir, Irem LU ; Fatih, Warda ; Svensson, Anja ; Radu, Dennis ; Linse, Sara LU ; Cabaleiro-Lago, Celia LU and Lundqvist, Martin LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 10
- issue
- 8
- article number
- e0136687
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:26313757
- wos:000360144000087
- scopus:84943233093
- pmid:26313757
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0136687
- language
- English
- LU publication?
- yes
- id
- d3627923-7e12-4d9c-8a46-6740e48a4660 (old id 7794134)
- date added to LUP
- 2016-04-01 13:21:06
- date last changed
- 2023-10-15 01:37:42
@article{d3627923-7e12-4d9c-8a46-6740e48a4660,
abstract = {{The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions.}},
author = {{Nasir, Irem and Fatih, Warda and Svensson, Anja and Radu, Dennis and Linse, Sara and Cabaleiro-Lago, Celia and Lundqvist, Martin}},
issn = {{1932-6203}},
language = {{eng}},
number = {{8}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{High Throughput Screening Method to Explore Protein Interactions with Nanoparticles.}},
url = {{http://dx.doi.org/10.1371/journal.pone.0136687}},
doi = {{10.1371/journal.pone.0136687}},
volume = {{10}},
year = {{2015}},
}