Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel
(2023) In FEMS Microbiology Letters 370.- Abstract
The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface is exposed to oxygen. Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. This study aimed to identify microbial genomes with metabolic potential for novel nitrogen- and metal-cycling reactions, representing biofilm microorganisms that could link these cycles and play a role in concrete biodeterioration. We reconstructed 33 abundant, novel... (More)
The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface is exposed to oxygen. Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. This study aimed to identify microbial genomes with metabolic potential for novel nitrogen- and metal-cycling reactions, representing biofilm microorganisms that could link these cycles and play a role in concrete biodeterioration. We reconstructed 33 abundant, novel metagenome-assembled genomes (MAGs) affiliated with the phylum Planctomycetota and candidate phylum KSB1. We identified novel and unusual genes and gene clusters in these MAGs related to anaerobic ammonium oxidation, nitrite oxidation, and other nitrogen-cycling reactions. Additionally, 26 of 33 MAGs also had the potential for iron, manganese and arsenite cycling, suggesting that bacteria represented by these genomes might couple these reactions. Our results expand the diversity of microorganisms putatively involved in nitrogen and metal cycling, and contribute to our understanding of potential biofilm impacts on built infrastructure.
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- author
- Suarez, Carolina LU ; Hackl, Thomas ; Wilen, Britt-Marie ; Persson, Frank ; Hagelia, Per ; Jetten, Mike and Martins, Paula Dalcin
- organization
- publishing date
- 2023-06-08
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Microbiology Letters
- volume
- 370
- publisher
- Oxford University Press
- external identifiers
-
- scopus:85164211815
- pmid:37291701
- ISSN
- 1574-6968
- DOI
- 10.1093/femsle/fnad049
- project
- Oslofjord tunnel microbiology
- language
- English
- LU publication?
- yes
- additional info
- © The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.
- id
- d3b0e159-db33-4f88-8f88-16cde4b135e0
- date added to LUP
- 2023-06-12 11:01:21
- date last changed
- 2024-04-19 22:46:12
@article{d3b0e159-db33-4f88-8f88-16cde4b135e0,
abstract = {{<p>The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface is exposed to oxygen. Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. This study aimed to identify microbial genomes with metabolic potential for novel nitrogen- and metal-cycling reactions, representing biofilm microorganisms that could link these cycles and play a role in concrete biodeterioration. We reconstructed 33 abundant, novel metagenome-assembled genomes (MAGs) affiliated with the phylum Planctomycetota and candidate phylum KSB1. We identified novel and unusual genes and gene clusters in these MAGs related to anaerobic ammonium oxidation, nitrite oxidation, and other nitrogen-cycling reactions. Additionally, 26 of 33 MAGs also had the potential for iron, manganese and arsenite cycling, suggesting that bacteria represented by these genomes might couple these reactions. Our results expand the diversity of microorganisms putatively involved in nitrogen and metal cycling, and contribute to our understanding of potential biofilm impacts on built infrastructure.</p>}},
author = {{Suarez, Carolina and Hackl, Thomas and Wilen, Britt-Marie and Persson, Frank and Hagelia, Per and Jetten, Mike and Martins, Paula Dalcin}},
issn = {{1574-6968}},
language = {{eng}},
month = {{06}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Letters}},
title = {{Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel}},
url = {{http://dx.doi.org/10.1093/femsle/fnad049}},
doi = {{10.1093/femsle/fnad049}},
volume = {{370}},
year = {{2023}},
}