Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Acquisition, maintenance and adaptation of invasion inhibitory antibodies against Plasmodium falciparum invasion ligands involved in immune evasion

Tijani, Muyideen K. ; Babalola, Oluwatoyin A. ; Odaibo, Alex B. ; Anumudu, Chiaka I. ; Asinobi, Adanze O. ; Morenikeji, Olajumoke A. ; Asuzu, Michael C. ; Langer, Christine ; Reiling, Linda and Beeson, James G. , et al. (2017) In PLoS ONE 12(8).
Abstract

Erythrocyte-binding antigens (EBAs) and P. falciparum reticulocyte-binding homologue proteins (PfRhs) are two important protein families that can vary in expression and utilization by P. falciparum to evade inhibitory antibodies. We evaluated antibodies at repeated time-points among individuals living in an endemic region in Nigeria over almost one year against these vaccine candidates. Antibody levels against EBA140, EBA175, EBA181, PfRh2, PfRh4, and MSP2, were measured by ELISA. We also used parasites with disrupted EBA140, EBA175 and EBA181 genes to show that all these were targets of invasion inhibitory antibodies. However, antigenic targets of inhibitory antibodies were not stable and changed substantially over time in most... (More)

Erythrocyte-binding antigens (EBAs) and P. falciparum reticulocyte-binding homologue proteins (PfRhs) are two important protein families that can vary in expression and utilization by P. falciparum to evade inhibitory antibodies. We evaluated antibodies at repeated time-points among individuals living in an endemic region in Nigeria over almost one year against these vaccine candidates. Antibody levels against EBA140, EBA175, EBA181, PfRh2, PfRh4, and MSP2, were measured by ELISA. We also used parasites with disrupted EBA140, EBA175 and EBA181 genes to show that all these were targets of invasion inhibitory antibodies. However, antigenic targets of inhibitory antibodies were not stable and changed substantially over time in most individuals, independent of age. Antibodies levels measured by ELISA also varied within and between individuals over time and the antibodies against EBA181, PfRh2 and MSP2 declined more rapidly in younger individuals (15 years) compared with older (>15). The breadth of high antibody responses over time was more influenced by age than by the frequency of infection. High antibody levels were associated with a more stable invasion inhibitory response, which could indicate that during the long process of formation of immunity, many changes not only in levels but also in functional responses are needed. This is an important finding in understanding natural immunity against malaria, which is essential for making an efficacious vaccine.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
12
issue
8
article number
e0182187
publisher
Public Library of Science (PLoS)
external identifiers
  • scopus:85026861473
  • pmid:28787025
  • wos:000406998800018
ISSN
1932-6203
DOI
10.1371/journal.pone.0182187
language
English
LU publication?
yes
id
d45cd4bf-c2a1-4e27-b5a0-f50c8103c3f1
date added to LUP
2017-08-23 15:24:32
date last changed
2025-01-07 19:13:14
@article{d45cd4bf-c2a1-4e27-b5a0-f50c8103c3f1,
  abstract     = {{<p>Erythrocyte-binding antigens (EBAs) and P. falciparum reticulocyte-binding homologue proteins (PfRhs) are two important protein families that can vary in expression and utilization by P. falciparum to evade inhibitory antibodies. We evaluated antibodies at repeated time-points among individuals living in an endemic region in Nigeria over almost one year against these vaccine candidates. Antibody levels against EBA140, EBA175, EBA181, PfRh2, PfRh4, and MSP2, were measured by ELISA. We also used parasites with disrupted EBA140, EBA175 and EBA181 genes to show that all these were targets of invasion inhibitory antibodies. However, antigenic targets of inhibitory antibodies were not stable and changed substantially over time in most individuals, independent of age. Antibodies levels measured by ELISA also varied within and between individuals over time and the antibodies against EBA181, PfRh2 and MSP2 declined more rapidly in younger individuals (15 years) compared with older (&gt;15). The breadth of high antibody responses over time was more influenced by age than by the frequency of infection. High antibody levels were associated with a more stable invasion inhibitory response, which could indicate that during the long process of formation of immunity, many changes not only in levels but also in functional responses are needed. This is an important finding in understanding natural immunity against malaria, which is essential for making an efficacious vaccine.</p>}},
  author       = {{Tijani, Muyideen K. and Babalola, Oluwatoyin A. and Odaibo, Alex B. and Anumudu, Chiaka I. and Asinobi, Adanze O. and Morenikeji, Olajumoke A. and Asuzu, Michael C. and Langer, Christine and Reiling, Linda and Beeson, James G. and Wahlgren, Mats and Nwuba, Roseangela I. and Persson, Kristina E.M.}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{8}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Acquisition, maintenance and adaptation of invasion inhibitory antibodies against Plasmodium falciparum invasion ligands involved in immune evasion}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0182187}},
  doi          = {{10.1371/journal.pone.0182187}},
  volume       = {{12}},
  year         = {{2017}},
}