Protocol for isolating nuclei from human stem cell-derived grafts for single-nucleus RNA sequencing
Sozzi, Edoardo LU
; Storm, Petter
LU
and Fiorenzano, Alessandro
LU
(2025)
In STAR Protocols
6(4).
- Abstract
Single-nucleus RNA sequencing enables high-resolution transcriptomic profiling of brain tissue, facilitating analysis of cell identity in models of neurodegeneration and repair. Here, we present a protocol for isolating nuclei from long-term human stem cell-derived grafts in the rat brain. We describe steps for vibratome sectioning, graft dissection, nuclear extraction, and fluorescence-activated sorting. This workflow supports analysis of human neurons embedded within host tissue or sensitive to dissociation, enabling assessment of graft composition, integration, and neuronal identity in the living brain. For complete details on the use and execution of this protocol, please refer to Fiorenzano et al.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d45daebd-0a90-4f78-920d-023308c3e035
- author
- Sozzi, Edoardo
LU
; Storm, Petter
LU
and Fiorenzano, Alessandro
LU
- organization
- publishing date
- 2025-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- STAR Protocols
- volume
- 6
- issue
- 4
- article number
- 104197
- publisher
- Cell Press
- external identifiers
-
- pmid:41206872
- scopus:105021092745
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2025.104197
- language
- English
- LU publication?
- yes
- id
- d45daebd-0a90-4f78-920d-023308c3e035
- date added to LUP
- 2025-12-08 12:31:00
- date last changed
- 2025-12-09 03:34:56
@article{d45daebd-0a90-4f78-920d-023308c3e035,
abstract = {{<p>Single-nucleus RNA sequencing enables high-resolution transcriptomic profiling of brain tissue, facilitating analysis of cell identity in models of neurodegeneration and repair. Here, we present a protocol for isolating nuclei from long-term human stem cell-derived grafts in the rat brain. We describe steps for vibratome sectioning, graft dissection, nuclear extraction, and fluorescence-activated sorting. This workflow supports analysis of human neurons embedded within host tissue or sensitive to dissociation, enabling assessment of graft composition, integration, and neuronal identity in the living brain. For complete details on the use and execution of this protocol, please refer to Fiorenzano et al.</p>}},
author = {{Sozzi, Edoardo and Storm, Petter and Fiorenzano, Alessandro}},
issn = {{2666-1667}},
language = {{eng}},
number = {{4}},
publisher = {{Cell Press}},
series = {{STAR Protocols}},
title = {{Protocol for isolating nuclei from human stem cell-derived grafts for single-nucleus RNA sequencing}},
url = {{http://dx.doi.org/10.1016/j.xpro.2025.104197}},
doi = {{10.1016/j.xpro.2025.104197}},
volume = {{6}},
year = {{2025}},
}