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Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance

Tognetti, Vanesa B; Van Aken, Olivier LU ; Morreel, Kris; Vandenbroucke, Korneel; van de Cotte, Brigitte; De Clercq, Inge; Chiwocha, Sheila; Fenske, Ricarda; Prinsen, Els and Boerjan, Wout, et al. (2010) In Plant Cell 22(8). p.79-2660
Abstract

Reactive oxygen species and redox signaling undergo synergistic and antagonistic interactions with phytohormones to regulate protective responses of plants against biotic and abiotic stresses. However, molecular insight into the nature of this crosstalk remains scarce. We demonstrate that the hydrogen peroxide-responsive UDP-glucosyltransferase UGT74E2 of Arabidopsis thaliana is involved in the modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Biochemical characterization of recombinant UGT74E2 demonstrated that it strongly favors IBA as a substrate. Assessment of indole-3-acetic acid (IAA), IBA, and their conjugates in transgenic plants ectopically expressing... (More)

Reactive oxygen species and redox signaling undergo synergistic and antagonistic interactions with phytohormones to regulate protective responses of plants against biotic and abiotic stresses. However, molecular insight into the nature of this crosstalk remains scarce. We demonstrate that the hydrogen peroxide-responsive UDP-glucosyltransferase UGT74E2 of Arabidopsis thaliana is involved in the modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Biochemical characterization of recombinant UGT74E2 demonstrated that it strongly favors IBA as a substrate. Assessment of indole-3-acetic acid (IAA), IBA, and their conjugates in transgenic plants ectopically expressing UGT74E2 indicated that the catalytic specificity was maintained in planta. In these transgenic plants, not only were IBA-Glc concentrations increased, but also free IBA levels were elevated and the conjugated IAA pattern was modified. This perturbed IBA and IAA homeostasis was associated with architectural changes, including increased shoot branching and altered rosette shape, and resulted in significantly improved survival during drought and salt stress treatments. Hence, our results reveal that IBA and IBA-Glc are important regulators of morphological and physiological stress adaptation mechanisms and provide molecular evidence for the interplay between hydrogen peroxide and auxin homeostasis through the action of an IBA UGT.

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keywords
Arabidopsis, Arabidopsis Proteins, Cloning, Molecular, Dehydration, Glucosyltransferases, Homeostasis, Indoleacetic Acids, Indoles, Mutagenesis, Insertional, Plants, Genetically Modified, Stress, Physiological
in
Plant Cell
volume
22
issue
8
pages
20 pages
publisher
American Society of Plant Biologists
external identifiers
  • scopus:77957813938
ISSN
1040-4651
DOI
10.1105/tpc.109.071316
language
English
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no
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d507c4fd-61d9-4595-9988-aba2cb33c39e
date added to LUP
2017-05-09 10:07:55
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2018-10-21 04:46:21
@article{d507c4fd-61d9-4595-9988-aba2cb33c39e,
  abstract     = {<p>Reactive oxygen species and redox signaling undergo synergistic and antagonistic interactions with phytohormones to regulate protective responses of plants against biotic and abiotic stresses. However, molecular insight into the nature of this crosstalk remains scarce. We demonstrate that the hydrogen peroxide-responsive UDP-glucosyltransferase UGT74E2 of Arabidopsis thaliana is involved in the modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Biochemical characterization of recombinant UGT74E2 demonstrated that it strongly favors IBA as a substrate. Assessment of indole-3-acetic acid (IAA), IBA, and their conjugates in transgenic plants ectopically expressing UGT74E2 indicated that the catalytic specificity was maintained in planta. In these transgenic plants, not only were IBA-Glc concentrations increased, but also free IBA levels were elevated and the conjugated IAA pattern was modified. This perturbed IBA and IAA homeostasis was associated with architectural changes, including increased shoot branching and altered rosette shape, and resulted in significantly improved survival during drought and salt stress treatments. Hence, our results reveal that IBA and IBA-Glc are important regulators of morphological and physiological stress adaptation mechanisms and provide molecular evidence for the interplay between hydrogen peroxide and auxin homeostasis through the action of an IBA UGT.</p>},
  author       = {Tognetti, Vanesa B and Van Aken, Olivier and Morreel, Kris and Vandenbroucke, Korneel and van de Cotte, Brigitte and De Clercq, Inge and Chiwocha, Sheila and Fenske, Ricarda and Prinsen, Els and Boerjan, Wout and Genty, Bernard and Stubbs, Keith A and Inzé, Dirk and Van Breusegem, Frank},
  issn         = {1040-4651},
  keyword      = {Arabidopsis,Arabidopsis Proteins,Cloning, Molecular,Dehydration,Glucosyltransferases,Homeostasis,Indoleacetic Acids,Indoles,Mutagenesis, Insertional,Plants, Genetically Modified,Stress, Physiological},
  language     = {eng},
  number       = {8},
  pages        = {79--2660},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Cell},
  title        = {Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance},
  url          = {http://dx.doi.org/10.1105/tpc.109.071316},
  volume       = {22},
  year         = {2010},
}