Advanced

The complementarity determining region 2 of BV8S2 (V beta 8.2) contributes to antigen recognition by rat invariant NKT cell TCR

Pyz, E; Naidenko, O; Miyake, S; Yamamura, T; Berberich, I; Cardell, Susanna LU ; Kronenberg, M and Herrmann, T (2006) In Journal of Immunology 176(12). p.7447-7455
Abstract
Invariant NKT cells (iNKT cells) are characterized by a semi-invariant TCR comprising an invariant a-chain paired with beta-chains with limited BV gene usage which are specific for complexes of CD1d and glycolipid Ags like a-galactosylceramide (a-GalCer). iNKT cells can be visualized with a-GalCer-loaded CD1d tetramers, and the binding of mouse CD1d tetramers to mouse as well as to human iNKT cells suggests a high degree of conservation in recognition of glycolipid Ags between species. Surprisingly, mouse CD1d tetramers failed to stain a discrete cell population among F344/Crl rat liver lymphocytes, although comprised iNKT cells are indicated by IL-4 and IFN-gamma secretion after a-GalCer stimulation. The arising hypothesis that rat iNKT... (More)
Invariant NKT cells (iNKT cells) are characterized by a semi-invariant TCR comprising an invariant a-chain paired with beta-chains with limited BV gene usage which are specific for complexes of CD1d and glycolipid Ags like a-galactosylceramide (a-GalCer). iNKT cells can be visualized with a-GalCer-loaded CD1d tetramers, and the binding of mouse CD1d tetramers to mouse as well as to human iNKT cells suggests a high degree of conservation in recognition of glycolipid Ags between species. Surprisingly, mouse CD1d tetramers failed to stain a discrete cell population among F344/Crl rat liver lymphocytes, although comprised iNKT cells are indicated by IL-4 and IFN-gamma secretion after a-GalCer stimulation. The arising hypothesis that rat iNKT TCR recognizes alpha-GalCer only if presented by syngeneic CD1d was then tested with the help of newly generated rat and mouse iNKT TCR-transduced cell lines. Cells expressing mouse iNKT TCR reacted to a-GalCer presented by rat or mouse CD1d and efficiently bound a-GalCer-loaded mouse CD1d tetramers. In contrast, cells expressing rat iNKT TCR responded only to a-GalCer presented by syngeneic CD1d and bound mouse CD1d tetramers only poorly or not at ail. Finally, CD1d-dependent a-GalCer reactivity and binding of mouse CD1d tetramers was tested for cells expressing iNKT TCR comprising either rat or mouse AV14 (V alpha 14) a-chains and wild-type or mutated BV8S2 (V beta 8.2) beta-chains. The results confirmed the need of syngeneic CD1d as restriction element for rat iNKT TCR and identified the CDR2 of BV8S2 as an essential site for ligand recognition by iNKT TCR. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
176
issue
12
pages
7447 - 7455
publisher
American Association of Immunologists
external identifiers
  • pmid:16751390
  • wos:000238101800036
  • scopus:33744943790
ISSN
1550-6606
language
English
LU publication?
yes
id
d52bb09c-86b9-48da-bbe0-92bc4b047b87 (old id 406783)
alternative location
http://www.jimmunol.org/cgi/content/abstract/176/12/7447
date added to LUP
2007-10-14 18:10:07
date last changed
2019-02-20 08:18:05
@article{d52bb09c-86b9-48da-bbe0-92bc4b047b87,
  abstract     = {Invariant NKT cells (iNKT cells) are characterized by a semi-invariant TCR comprising an invariant a-chain paired with beta-chains with limited BV gene usage which are specific for complexes of CD1d and glycolipid Ags like a-galactosylceramide (a-GalCer). iNKT cells can be visualized with a-GalCer-loaded CD1d tetramers, and the binding of mouse CD1d tetramers to mouse as well as to human iNKT cells suggests a high degree of conservation in recognition of glycolipid Ags between species. Surprisingly, mouse CD1d tetramers failed to stain a discrete cell population among F344/Crl rat liver lymphocytes, although comprised iNKT cells are indicated by IL-4 and IFN-gamma secretion after a-GalCer stimulation. The arising hypothesis that rat iNKT TCR recognizes alpha-GalCer only if presented by syngeneic CD1d was then tested with the help of newly generated rat and mouse iNKT TCR-transduced cell lines. Cells expressing mouse iNKT TCR reacted to a-GalCer presented by rat or mouse CD1d and efficiently bound a-GalCer-loaded mouse CD1d tetramers. In contrast, cells expressing rat iNKT TCR responded only to a-GalCer presented by syngeneic CD1d and bound mouse CD1d tetramers only poorly or not at ail. Finally, CD1d-dependent a-GalCer reactivity and binding of mouse CD1d tetramers was tested for cells expressing iNKT TCR comprising either rat or mouse AV14 (V alpha 14) a-chains and wild-type or mutated BV8S2 (V beta 8.2) beta-chains. The results confirmed the need of syngeneic CD1d as restriction element for rat iNKT TCR and identified the CDR2 of BV8S2 as an essential site for ligand recognition by iNKT TCR.},
  author       = {Pyz, E and Naidenko, O and Miyake, S and Yamamura, T and Berberich, I and Cardell, Susanna and Kronenberg, M and Herrmann, T},
  issn         = {1550-6606},
  language     = {eng},
  number       = {12},
  pages        = {7447--7455},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {The complementarity determining region 2 of BV8S2 (V beta 8.2) contributes to antigen recognition by rat invariant NKT cell TCR},
  volume       = {176},
  year         = {2006},
}