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Flow cytometric analysis of myelodysplasia : Pre-analytical and technical issues—Recommendations from the European LeukemiaNet

van der Velden, Vincent H.J. ; Preijers, Frank ; Johansson, Ulrika ; Westers, Theresia M. ; Dunlop, Alan ; Porwit, Anna LU ; Béné, Marie C. ; Valent, Peter ; te Marvelde, Jeroen and Wagner-Ballon, Orianne , et al. (2023) In Cytometry Part B - Clinical Cytometry 104(1). p.15-26
Abstract

Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions: These recommendations... (More)

Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ELN, flow cytometry, MDS, pre-analytic issues
in
Cytometry Part B - Clinical Cytometry
volume
104
issue
1
pages
15 - 26
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:34894176
  • scopus:85120851920
  • scopus:85128496758
ISSN
1552-4949
DOI
10.1002/cyto.b.22046
language
English
LU publication?
yes
id
d54c53f1-9704-446c-b9f5-e91f5723f4be
date added to LUP
2022-02-07 14:24:36
date last changed
2024-04-04 03:20:15
@article{d54c53f1-9704-446c-b9f5-e91f5723f4be,
  abstract     = {{<p>Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.</p>}},
  author       = {{van der Velden, Vincent H.J. and Preijers, Frank and Johansson, Ulrika and Westers, Theresia M. and Dunlop, Alan and Porwit, Anna and Béné, Marie C. and Valent, Peter and te Marvelde, Jeroen and Wagner-Ballon, Orianne and Oelschlaegel, Uta and Saft, Leonie and Kordasti, Sharham and Ireland, Robin and Cremers, Eline and Alhan, Canan and Duetz, Carolien and Hobo, Willemijn and Chapuis, Nicolas and Fontenay, Michaela and Bettelheim, Peter and Eidenshink-Brodersen, Lisa and Font, Patricia and Loken, Michael R. and Matarraz, Sergio and Ogata, Kiyoyuki and Orfao, Alberto and Psarra, Katherina and Subirá, Dolores and Wells, Denise A. and Della Porta, Matteo G. and Burbury, Kate and Bellos, Frauke and Weiß, Elisabeth and Kern, Wolfgang and van de Loosdrecht, Arjan}},
  issn         = {{1552-4949}},
  keywords     = {{ELN; flow cytometry; MDS; pre-analytic issues}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{15--26}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Cytometry Part B - Clinical Cytometry}},
  title        = {{Flow cytometric analysis of myelodysplasia : Pre-analytical and technical issues—Recommendations from the European LeukemiaNet}},
  url          = {{http://dx.doi.org/10.1002/cyto.b.22046}},
  doi          = {{10.1002/cyto.b.22046}},
  volume       = {{104}},
  year         = {{2023}},
}