Advanced

A phylogenomic tree inferred with an inexpensive PCR-generated probe kit resolves higher-level relationships among Neptis butterflies (Nymphalidae : Limenitidinae)

Ma, Lijun ; Zhang, Yuan ; Lohman, David J. ; Wahlberg, Niklas LU ; Ma, Fangzhou ; Nylin, Soren ; Janz, Niklas ; Yago, Masaya ; Aduse-Poku, Kwaku and Peggie, Djunijanti , et al. (2020) In Systematic Entomology
Abstract

Recent advances in obtaining reduced representation libraries for next-generation sequencing permit phylogenomic analysis of species-rich, recently diverged taxa. In this study, we performed sequence capture with homemade PCR-generated probes to study diversification among closely related species in a large insect genus to examine the utility of this method. We reconstructed the phylogeny of Neptis Fabricius, a large and poorly studied nymphalid butterfly genus distributed throughout the Old World. We inferred relationships among 108 Neptis samples using 89 loci totaling up to 84 519 bp per specimen. Our taxon sample focused on Palearctic, Oriental and Australasian species, but included 8 African species and outgroups from 5 related... (More)

Recent advances in obtaining reduced representation libraries for next-generation sequencing permit phylogenomic analysis of species-rich, recently diverged taxa. In this study, we performed sequence capture with homemade PCR-generated probes to study diversification among closely related species in a large insect genus to examine the utility of this method. We reconstructed the phylogeny of Neptis Fabricius, a large and poorly studied nymphalid butterfly genus distributed throughout the Old World. We inferred relationships among 108 Neptis samples using 89 loci totaling up to 84 519 bp per specimen. Our taxon sample focused on Palearctic, Oriental and Australasian species, but included 8 African species and outgroups from 5 related genera. Maximum likelihood and Bayesian analyses yielded identical trees with full support for almost all nodes. We confirmed that Neptis is not monophyletic because Lasippa heliodore (Fabricius) and Phaedyma amphion (Linnaeus) are nested within the genus, and we redefine species groups for Neptis found outside of Africa. The statistical support of our results demonstrates that the probe set we employed is useful for inferring phylogenetic relationships among Neptis species and likely has great value for intrageneric phylogenetic reconstruction of Lepidoptera. Based on our results, we revise the following two taxa: Neptis heliodore comb. rev. and Neptis amphion comb. rev.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Systematic Entomology
publisher
Wiley-Blackwell
external identifiers
  • scopus:85085499902
ISSN
0307-6970
DOI
10.1111/syen.12435
language
English
LU publication?
yes
id
d57ff309-808a-47fa-b234-3fcd823b0e47
date added to LUP
2020-06-18 13:22:16
date last changed
2020-08-26 20:17:54
@article{d57ff309-808a-47fa-b234-3fcd823b0e47,
  abstract     = {<p>Recent advances in obtaining reduced representation libraries for next-generation sequencing permit phylogenomic analysis of species-rich, recently diverged taxa. In this study, we performed sequence capture with homemade PCR-generated probes to study diversification among closely related species in a large insect genus to examine the utility of this method. We reconstructed the phylogeny of Neptis Fabricius, a large and poorly studied nymphalid butterfly genus distributed throughout the Old World. We inferred relationships among 108 Neptis samples using 89 loci totaling up to 84 519 bp per specimen. Our taxon sample focused on Palearctic, Oriental and Australasian species, but included 8 African species and outgroups from 5 related genera. Maximum likelihood and Bayesian analyses yielded identical trees with full support for almost all nodes. We confirmed that Neptis is not monophyletic because Lasippa heliodore (Fabricius) and Phaedyma amphion (Linnaeus) are nested within the genus, and we redefine species groups for Neptis found outside of Africa. The statistical support of our results demonstrates that the probe set we employed is useful for inferring phylogenetic relationships among Neptis species and likely has great value for intrageneric phylogenetic reconstruction of Lepidoptera. Based on our results, we revise the following two taxa: Neptis heliodore comb. rev. and Neptis amphion comb. rev.</p>},
  author       = {Ma, Lijun and Zhang, Yuan and Lohman, David J. and Wahlberg, Niklas and Ma, Fangzhou and Nylin, Soren and Janz, Niklas and Yago, Masaya and Aduse-Poku, Kwaku and Peggie, Djunijanti and Wang, Min and Zhang, Peng and Wang, Houshuai},
  issn         = {0307-6970},
  language     = {eng},
  month        = {05},
  publisher    = {Wiley-Blackwell},
  series       = {Systematic Entomology},
  title        = {A phylogenomic tree inferred with an inexpensive PCR-generated probe kit resolves higher-level relationships among Neptis butterflies (Nymphalidae : Limenitidinae)},
  url          = {http://dx.doi.org/10.1111/syen.12435},
  doi          = {10.1111/syen.12435},
  year         = {2020},
}