Ultrastructure and phylogenetic position of Regin rotiferus and Otto terricolus genera et species novae (Bicosoecida, Heterokonta/Stramenopiles)
(2014) In Protist 165(2). p.144-160- Abstract
We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terricolus displays unique novel morphological traits; thus, it has a basal swelling on each flagellum, a root 3/root 2 distribution of 10 + 1 microtubules, and an amoeboid stage in its life cycle. Regin rotiferus has flagella without swellings and a root 3/root 2 distribution of 7 + 3 microtubules, a pattern commonly observed in the Bicosoecida. We... (More)
We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terricolus displays unique novel morphological traits; thus, it has a basal swelling on each flagellum, a root 3/root 2 distribution of 10 + 1 microtubules, and an amoeboid stage in its life cycle. Regin rotiferus has flagella without swellings and a root 3/root 2 distribution of 7 + 3 microtubules, a pattern commonly observed in the Bicosoecida. We present an updated exhaustive maximum likelihood phylogeny of 48 cultured, complete or nearly complete (+1600 bp) 18S rDNA Bicosoecida sequences. Both new species fall into a well-supported freshwater Siluaniidae clade, without being particularly closely related. The morphology and phylogeny do not conclusively support Rictus as a member of Bicosoecida.
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- author
- Harder, Christoffer Bugge LU ; Ekelund, Flemming and Karpov, Sergey A
- publishing date
- 2014-03
- type
- Contribution to journal
- publication status
- published
- keywords
- Cluster Analysis, DNA, Algal/chemistry, DNA, Ribosomal/chemistry, DNA, Ribosomal Spacer/genetics, Microscopy, Organelles/ultrastructure, Phylogeny, RNA, Ribosomal, 18S/genetics, Sequence Analysis, DNA, Soil/parasitology, Stramenopiles/classification
- in
- Protist
- volume
- 165
- issue
- 2
- pages
- 144 - 160
- publisher
- Elsevier
- external identifiers
-
- scopus:84896136095
- pmid:24637333
- ISSN
- 1434-4610
- DOI
- 10.1016/j.protis.2014.01.004
- language
- English
- LU publication?
- no
- additional info
- Copyright © 2014 Elsevier GmbH. All rights reserved.
- id
- d5ea88aa-31c4-4584-8eb3-dc30882de24d
- date added to LUP
- 2020-09-09 11:15:36
- date last changed
- 2025-01-10 19:36:05
@article{d5ea88aa-31c4-4584-8eb3-dc30882de24d,
abstract = {{<p>We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terricolus displays unique novel morphological traits; thus, it has a basal swelling on each flagellum, a root 3/root 2 distribution of 10 + 1 microtubules, and an amoeboid stage in its life cycle. Regin rotiferus has flagella without swellings and a root 3/root 2 distribution of 7 + 3 microtubules, a pattern commonly observed in the Bicosoecida. We present an updated exhaustive maximum likelihood phylogeny of 48 cultured, complete or nearly complete (+1600 bp) 18S rDNA Bicosoecida sequences. Both new species fall into a well-supported freshwater Siluaniidae clade, without being particularly closely related. The morphology and phylogeny do not conclusively support Rictus as a member of Bicosoecida.</p>}},
author = {{Harder, Christoffer Bugge and Ekelund, Flemming and Karpov, Sergey A}},
issn = {{1434-4610}},
keywords = {{Cluster Analysis; DNA, Algal/chemistry; DNA, Ribosomal/chemistry; DNA, Ribosomal Spacer/genetics; Microscopy; Organelles/ultrastructure; Phylogeny; RNA, Ribosomal, 18S/genetics; Sequence Analysis, DNA; Soil/parasitology; Stramenopiles/classification}},
language = {{eng}},
number = {{2}},
pages = {{144--160}},
publisher = {{Elsevier}},
series = {{Protist}},
title = {{Ultrastructure and phylogenetic position of Regin rotiferus and Otto terricolus genera et species novae (Bicosoecida, Heterokonta/Stramenopiles)}},
url = {{http://dx.doi.org/10.1016/j.protis.2014.01.004}},
doi = {{10.1016/j.protis.2014.01.004}},
volume = {{165}},
year = {{2014}},
}