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Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015

Lenman, Marit LU ; Ali, Ashfaq LU orcid ; Mühlenbock, Per ; Carlson-Nilsson, Ulrika ; Liljeroth, Erland ; Champouret, Nicolas ; Vleeshouwers, Vivianne G A A and Andreasson, Erik LU (2016) In Theoretical And Applied Genetics 129(1). p.15-105
Abstract

KEY MESSAGE: We show the usefulness of integrating effector screening in a breeding program and in resistance gene cloning, with Phytophthora resistance in the Swedish potato breeding clone SW93-1015 as an example. Phytophthora infestans is one of the most devastating plant pathogens worldwide. We have earlier found that the SW93-1015 potato breeding clone has an efficient resistance against P. infestans under field conditions in Sweden, which has an unusually high local diversity of the pathogen. This potato clone has characteristics that are different from classical R-gene-mediated resistance such as elevated levels of hydrogen peroxide (H2O2) under controlled conditions. Analysis of 76 F1 potato progenies from two individual crosses... (More)

KEY MESSAGE: We show the usefulness of integrating effector screening in a breeding program and in resistance gene cloning, with Phytophthora resistance in the Swedish potato breeding clone SW93-1015 as an example. Phytophthora infestans is one of the most devastating plant pathogens worldwide. We have earlier found that the SW93-1015 potato breeding clone has an efficient resistance against P. infestans under field conditions in Sweden, which has an unusually high local diversity of the pathogen. This potato clone has characteristics that are different from classical R-gene-mediated resistance such as elevated levels of hydrogen peroxide (H2O2) under controlled conditions. Analysis of 76 F1 potato progenies from two individual crosses resulted in nearly 50% resistant clones, from both crosses. This result suggests that the SW93-1015 clone has a simplex genotype for this trait. Screening with over 50 different P. infestans effectors, containing the conserved motif RXLR (for Arg, any amino acid, Leu, Arg), revealed a specific response to Avr2, which suggests that SW93-1015 might contain a functional homolog of the R2 resistance gene. We cloned eight R2 gene homologs from SW93-1015, whereof seven have not been described before and one gene encoded a protein identical to Rpi-ABPT. Expression of this gene in potato cultivar Désirée provided R2-specific resistance, whereas other homologues did not. Using RNAseq analyses we designed a new DNA marker for the R2 resistance in SW93-1015. In summary, we have demonstrated the use of effector screening in practical breeding material and revealed the key resistance mechanism for SW93-1015.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Breeding, Cloning, Molecular, Disease Resistance/genetics, Genes, Plant, Genetic Markers, Genotype, Molecular Sequence Data, Phytophthora infestans, Plant Diseases/genetics, Plants, Genetically Modified/genetics, Solanum tuberosum/genetics
in
Theoretical And Applied Genetics
volume
129
issue
1
pages
11 pages
publisher
Springer
external identifiers
  • scopus:84953355769
  • pmid:26518573
ISSN
0040-5752
DOI
10.1007/s00122-015-2613-y
language
English
LU publication?
no
id
d6a4b8a8-3b5b-4957-92c1-30c66b544ad5
date added to LUP
2020-03-30 16:05:30
date last changed
2024-01-16 23:53:54
@article{d6a4b8a8-3b5b-4957-92c1-30c66b544ad5,
  abstract     = {{<p>KEY MESSAGE: We show the usefulness of integrating effector screening in a breeding program and in resistance gene cloning, with Phytophthora resistance in the Swedish potato breeding clone SW93-1015 as an example. Phytophthora infestans is one of the most devastating plant pathogens worldwide. We have earlier found that the SW93-1015 potato breeding clone has an efficient resistance against P. infestans under field conditions in Sweden, which has an unusually high local diversity of the pathogen. This potato clone has characteristics that are different from classical R-gene-mediated resistance such as elevated levels of hydrogen peroxide (H2O2) under controlled conditions. Analysis of 76 F1 potato progenies from two individual crosses resulted in nearly 50% resistant clones, from both crosses. This result suggests that the SW93-1015 clone has a simplex genotype for this trait. Screening with over 50 different P. infestans effectors, containing the conserved motif RXLR (for Arg, any amino acid, Leu, Arg), revealed a specific response to Avr2, which suggests that SW93-1015 might contain a functional homolog of the R2 resistance gene. We cloned eight R2 gene homologs from SW93-1015, whereof seven have not been described before and one gene encoded a protein identical to Rpi-ABPT. Expression of this gene in potato cultivar Désirée provided R2-specific resistance, whereas other homologues did not. Using RNAseq analyses we designed a new DNA marker for the R2 resistance in SW93-1015. In summary, we have demonstrated the use of effector screening in practical breeding material and revealed the key resistance mechanism for SW93-1015.</p>}},
  author       = {{Lenman, Marit and Ali, Ashfaq and Mühlenbock, Per and Carlson-Nilsson, Ulrika and Liljeroth, Erland and Champouret, Nicolas and Vleeshouwers, Vivianne G A A and Andreasson, Erik}},
  issn         = {{0040-5752}},
  keywords     = {{Amino Acid Sequence; Breeding; Cloning, Molecular; Disease Resistance/genetics; Genes, Plant; Genetic Markers; Genotype; Molecular Sequence Data; Phytophthora infestans; Plant Diseases/genetics; Plants, Genetically Modified/genetics; Solanum tuberosum/genetics}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{15--105}},
  publisher    = {{Springer}},
  series       = {{Theoretical And Applied Genetics}},
  title        = {{Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015}},
  url          = {{http://dx.doi.org/10.1007/s00122-015-2613-y}},
  doi          = {{10.1007/s00122-015-2613-y}},
  volume       = {{129}},
  year         = {{2016}},
}